Simple synthesis of functionalized superparamagnetic magnetite/silica core/shell nanoparticles and their application as magnetically separable high-performance biocatalysts
We report on the facile large-scale synthesis of magnetite@silica core-shell nanoparticles by a simple addition of tetraethyl orthosilicate (TEOS) into reverse micelles during the formation of uniformly-sized magnetite nanoparticles. The size of magnetic core was determined by the ratio of solvent and surfactant in reverse micelle solution while the thickness of silica shell could be easily controlled by adjusting the amount of added TEOS. Amino group functional groups were grafted to the magnetic nanoparticles, and crosslinked enzyme clusters (CEC) were fabricated on the surface of magnetite@silica nanoparticles. The resulting hybrid materials of magnetite and CEC were magnetically separable, highly active, and stable enough to show no decrease of enzyme activity under rigorous shaking for more than 15 days.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 951038
- Report Number(s):
- PNNL-SA-51532; 25404; 3241; 400403809
- Journal Information:
- Small, 4(1):143-152, Journal Name: Small, 4(1):143-152 Journal Issue: 1 Vol. 4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
77 NANOSCIENCE AND NANOTECHNOLOGY
CATALYSTS
ENZYME ACTIVITY
ENZYMES
Environmental Molecular Sciences Laboratory
MAGNETITE
MICELLAR SYSTEMS
NANOSTRUCTURES
PARAMAGNETISM
SILICA
STABILITY
SYNTHESIS
enzyme stabilization
crosslinked enzyme clusters
magnetic nanoparticles
lipase and chymotrypsin