Proteome web : a web-based interface for the display and interrogation of proteomes.

Journal Article · Thu May 01 04:00:00 EDT 2003 · Proteomics

DOI:https://doi.org/10.1002/pmic.200300396· OSTI ID:949603

Babnigg, G; Giometti, C S

The analysis of proteomes, i.e., the proteins expressed by biological organisms under a given set of conditions at a given time, requires separating complex protein mixtures into discrete protein components, measuring their relative abundances, and identifying the individual protein components. Many types of data are generated during the course of proteome analysis, including graphic images of the protein profiles, flat files containing numeric data, spreadsheets for assimilating numeric data, and relational database tables for integrating data from multiple experiments. As part of a project to describe the proteomes of microbes of interest to the U.S. Department of Energy, a World-Wide Web-based interface has been developed for the display of protein profiles generated by two-dimensional gel electrophoresis. The web interface is capable of obtaining protein identifications on the fly, interrogating the quantitative data in the context of available genome sequence information, and relating the proteome data to existing metabolic pathway databases. Analysis of protein expression profiles is expedited, providing the capability to efficiently determine the gene locations for proteins modulated in abundance in response to different growth conditions and to locate the positions of the proteins within specific metabolic pathways. The proteome of the archaeon Methanococcus jannaschii, a microbe for which the complete genome sequence is available, is used to demonstrate the capabilities of this evolving web interface (http://proteomeweb.anl.gov).

• Research Organization: Argonne National Laboratory (ANL)
• Sponsoring Organization: SC
• DOE Contract Number: AC02-06CH11357
• OSTI ID: 949603
• Report Number(s): ANL/BIO/JA-42449
• Journal Information: Proteomics, Journal Name: Proteomics Journal Issue: 5 ; May, 2003 Vol. 3
• Country of Publication: United States
• Language: ENGLISH

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS
ABUNDANCE
BIOLOGICAL PATHWAYS
COMMUNICATIONS
COMPUTERS
ELECTROPHORESIS
GENES
INTERNET
MIXTURES
PROTEINS