X-ray absorption fine-structure determination of pH-dependent U-bacterial cell wall interactions.

Journal Article · Tue Jan 01 04:00:00 EST 2002 · Geochim. Cosmochim. Acta

DOI:https://doi.org/10.1016/S0016-7037(02)00947-X· OSTI ID:949419

Kelly, S D; Kemner, K M; Fein, J B; Fowle, D A; Boyanov, M I; Bunker, B A; Yee, N

X-ray absorption fine structure (XAFS) measurements was used at the U L3-edge to directly determine the pH dependence of the cell wall functional groups responsible for the absorption of aqueous UO{sub 2}{sup 2+} to Bacillus subtilis from pH 1.67 to 4.80. Surface complexation modeling can be used to predict metal distributions in water-rock systems, and it has been used to quantify bacterial adsorption of metal cations. However, successful application of these models requires a detailed knowledge not only of the type of bacterial surface site involved in metal adsorption/desorption, but also of the binding geometry. Previous acid-base titrations of B. subtilis cells suggested that three surface functional group types are important on the cell wall; these groups have been postulated to correspond to carboxyl, phosphoryl, and hydroxyl sites. When the U(VI) adsorption to B. subtilis is measured, observed is a significant pH-independent absorption at low pH values (<3.0), ascribed to an interaction between the uranyl cation and a neutrally charged phosphoryl group on the cell wall. The present study provides independent quantitative constraints on the types of sites involved in uranyl binding to B. subtilis from pH 1.67 to 4.80. The XAFS results indicate that at extremely low pH (pH 1.67) UO{sub 2}{sup 2+} binds exclusively to phosphoryl functional groups on the cell wall, with an average distance between the U atom and the P atom of 3.64 {+-} 0.01 Angstroms. This U-P distance indicates an inner-sphere complex with an oxygen atom shared between the UO{sub 2}{sup 2+} and the phosphoryl ligand. The P signal at extremely low pH value is consistent with the UO{sub 2}{sup 2+} binding to a protonated phosphoryl group, as previously ascribed. With increasing pH (3.22 and 4.80), UO{sub 2}{sup 2+} binds increasingly to bacterial surface carboxyl functional groups, with an average distance between the U atom and the C atom of 2.89 {+-} 0.02 Angstroms. This U-C distance indicates an inner-sphere complex with two oxygen atoms shared between the UO{sub 2}{sup 2+} and the carboxyl ligand. The results of this XAFS study confirm the uranyl-bacterial surface speciation model.

• Research Organization: Argonne National Laboratory (ANL)
• Sponsoring Organization: SC; NSF; OUS
• DOE Contract Number: AC02-06CH11357
• OSTI ID: 949419
• Report Number(s): ANL/ER/JA-40750
• Journal Information: Geochim. Cosmochim. Acta, Journal Name: Geochim. Cosmochim. Acta Journal Issue: 22 ; 2002 Vol. 66; ISSN GCACAK; ISSN 0016-7037
• Country of Publication: United States
• Language: ENGLISH

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
99 GENERAL AND MISCELLANEOUS
ABSORPTION
ADSORPTION
ATOMS
BACILLUS SUBTILIS
CATIONS
CELL WALL
FINE STRUCTURE
FUNCTIONALS
GEOMETRY
OXYGEN
PH VALUE
SIMULATION
X-RAY SPECTROSCOPY