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Comparison of PAH Biodegradation and Desorption Kinetics During Bioremediation of Aged Petroleum Hydrocarbon Contaminated Soils

Conference ·
OSTI ID:947953
It is commonly assumed that mass-transfer limitations are the cause for slow and incomplete biodegradation of PAHs in aged soils. In order to test this hypothesis, the biodegradation rate and the abiotic release rate were measured and compared for selected PAHs in three different soils. It was found that PAH biodegradation was not mass-transfer limited during slurry bioremediation of an aged loamy soil. By contrast, PAH biodegradation rates were much larger than abiotic release rates in kaolinite clay indicating that sorbed-phase PAHs can apparently be biodegraded directly from mineral surfaces without prior desorption or dissolution into the aqueous phase. A comparison of PAH biodegradation rates and abiotic release rates at termination of the slurry bioremediation treatment revealed that abiotic release rates are much larger than the respective biodegradation rates. In addition, it was found that the number of hydrocarbon degraders decreased by four orders of magnitude during the bioremediation treatment. It can therefore be concluded that the slow and incomplete biodegradation of PAHs is not caused by mass-transfer limitations but rather by microbial factors. Consequently, the residual PAHs that remain after extensive bioremediation treatment are still bioavailable and for that reason could pose a greater risk to environmental receptors than previously thought.
Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-76RL01830
OSTI ID:
947953
Report Number(s):
PNNL-SA-33409; KP1301010
Country of Publication:
United States
Language:
English