Adjustable virtual pore-size filter for automated sample preparation using acoustic radiation force

Journal Article · Thu May 22 04:00:00 EDT 2008 · Analytical Chemistry, vol. 80, N/A, October 11, 2008, pp. 8447-8452

OSTI ID:945697

Jung, B; Fisher, K; Ness, K; Rose, K; Mariella, R

We present a rapid and robust size-based separation method for high throughput microfluidic devices using acoustic radiation force. We developed a finite element modeling tool to predict the two-dimensional acoustic radiation force field perpendicular to the flow direction in microfluidic devices. Here we compare the results from this model with experimental parametric studies including variations of the PZT driving frequencies and voltages as well as various particle sizes and compressidensities. These experimental parametric studies also provide insight into the development of an adjustable 'virtual' pore-size filter as well as optimal operating conditions for various microparticle sizes. We demonstrated the separation of Saccharomyces cerevisiae and MS2 bacteriophage using acoustic focusing. The acoustic radiation force did not affect the MS2 viruses, and their concentration profile remained unchanged. With optimized design of our microfluidic flow system we were able to achieve yields of > 90% for the MS2 with > 80% of the S. cerevisiae being removed in this continuous-flow sample preparation device.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA
• Sponsoring Organization: USDOE
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 945697
• Report Number(s): LLNL-JRNL-404158
• Journal Information: Analytical Chemistry, vol. 80, N/A, October 11, 2008, pp. 8447-8452, Journal Name: Analytical Chemistry, vol. 80, N/A, October 11, 2008, pp. 8447-8452 Vol. 80
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ACOUSTICS
BACTERIOPHAGES
DESIGN
FOCUSING
PARTICLE SIZE
RADIATIONS
SACCHAROMYCES CEREVISIAE
SAMPLE PREPARATION
SIMULATION
VIRUSES