Proteomic Analysis of and Immune Responses to Ehrlichia chaffeensis Lipoproteins
Journal Article
·
· Infection and Immunity, 76(8):3405-3414
Ehrlichia chaffeensis is an obligatory intracellular Gram-negative bacterium and is the etiologic agent of human monocytic ehrlichiosis (HME). Although E. chaffeensis induces generation of several cytokines and chemokines by leukocytes, E. chaffeensis lacks LPS and peptidoglycan. Bioinfomatic analysis of the E. chaffeensis genome, however, predicted genes encoding fifteen lipoproteins and three posttranslational lipoprotein processing enzymes. The present study showed that using multidimensional liquid chromatography followed by tandem mass spectrometry, all predicted lipoproteins as well as lipoprotein-processing enzymes were expressed by E. chaffeensis cultured in human promyelocytic leukemia cell line, HL-60. Consistent with this observation, a signal peptidase II inhibitor, globomycin was found to inhibit E. chaffeensis infection and lipoprotein processing in HL-60 cell culture. To study in vivo E.chaffeensis lipoprotein expression and host immune responses to E. chaffeensis lipoproteins, thirteen E. chaffeensis lipoprotein genes were cloned into a mammalian expression vector.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 944894
- Report Number(s):
- PNNL-SA-60631; 25412; 15898; KP1504020
- Journal Information:
- Infection and Immunity, 76(8):3405-3414, Journal Name: Infection and Immunity, 76(8):3405-3414 Journal Issue: 8 Vol. 76; ISSN 0019-9567; ISSN INFIBR
- Country of Publication:
- United States
- Language:
- English
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