Metal Reduction and Iron Biomineralization by a Psychrotolerant Fe(III)-Reducing Bacterium, Shewanella sp. Strain PV-4

Journal Article · Mon May 01 04:00:00 EDT 2006 · Applied and Environmental Microbiology, 72(5):3236-3244

DOI:https://doi.org/10.1128/AEM.72.5.3236-3244.2006· OSTI ID:944788

Roh, Yul; Gao, Haichun; Vali, Hojatollah; Kennedy, David W; Yang, Zamin; Gao, Weimin; Dohnalkova, Alice; Stapleton, Raymond D; Moon, Ji-Won; Phelps, T J; Fredrickson, Jim K; Zhou, Jizhong

A marine psychrotolerant, dissimilatory Fe(III)-reducing bacterium, Shewanella sp. strain PV-4, from the microbial mat at a hydrothermal vent of Loihi Seamount in the Pacific Ocean has been further characterized, with emphases on metal reduction and iron biomineralization. The strain is able to reduce metals such as Fe(III), Co(III), Cr(VI), Mn(IV), and U(VI) as electron acceptors while using lactate, formate, pyruvate, or hydrogen as an electron donor. Growth during iron reduction occurred over the pH range of 7.0 to 8.9, a sodium chloride range of 0.05 to 5%, and a temperature range of 0 to 37°C, with an optimum growth temperature of 18°C. Unlike mesophilic dissimilatory Fe(III)-reducing bacteria, which produce mostly superparamagnetic magnetite (<35 nm), this psychrotolerant bacterium produces well-formed single-domain magnetite (>35 nm) at temperatures from 18 to 37°C. The genome size of this strain is about 4.5 Mb. Strain PV-4 is sensitive to a variety of commonly used antibiotics except ampicillin and can acquire exogenous DNA (plasmid pCM157) through conjugation.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 944788
• Report Number(s): PNNL-SA-52231; KP1501021
• Journal Information: Applied and Environmental Microbiology, 72(5):3236-3244, Journal Name: Applied and Environmental Microbiology, 72(5):3236-3244
• Country of Publication: United States
• Language: English

Related Subjects

Loihi Seamount
Shewanella sp. strain PV-4
dissimilatory Fe(III)-reducing bacterium
marine psychrotolerant
psychrotolerant bacterium
superparamagnetic magnetite