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An inexpensive fathead minnow egg incubation and toxicant exposure system

Journal Article · · Environmental Toxicology and Chemistry
; ;  [1]
  1. Miami Univ., Oxford, OH (United States). Dept. of Zoology

Several methods have been developed for bulk hatching of fathead minnow eggs for laboratory and commercial culture. These methods generally involve placing whole, egg-laden breeding substrates in an aeration or water-flow device, or manually removing eggs from breeding substrates. Eggs removed from substrates are then hatched in Downing or McDonald jar hatching devices or are agitated in cylindrical vessels from which larvae are manually removed. These methods are difficult to incorporate into toxicity tests involving determination of hatching success in replicate systems. Both require either continuous water flow to individual hatching chambers or frequent static renewal, which adds to the labor of separating larvae from unhatched eggs. The authors report on an inexpensive, easily constructed system for hatching fathead minnow eggs and maintaining hatched larvae for growth and survivorship studies. Data are presented to illustrate the use of the system for toxicant exposures. This system has applications for both field and laboratory studies.

Sponsoring Organization:
USDOE
OSTI ID:
94318
Journal Information:
Environmental Toxicology and Chemistry, Journal Name: Environmental Toxicology and Chemistry Journal Issue: 8 Vol. 14; ISSN ETOCDK; ISSN 0730-7268
Country of Publication:
United States
Language:
English

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