Massive parallel analysis of DNA - Hoechst 33258 binding specificity with a generic oligonucleotide microchip.
A generic oligodeoxyribonucleotide microchip was used to determine the sequence specificity of Hoechst 33258 binding to double-stranded DNA. The generic microchip contained 4096 oxctadeoxynucleo-tides in which all possible 4(6)= 4096 hexadeoxy-nucleotide sequences are flanked on both the 3'- and 5'-ends with equimolar mixtures of four bases. The microchip was manufactured by chemical immobilization of presynthesized 8mers within polyacrylamide gel pads. A selected set of immobilized 8mers was converted to double-stranded form by hybridization with a mixture of fluorescently labeled complementary 8mers. Massive parallel measurements of melting curves were carried out for the majority of 2080 6mer duplexes, in both the absence and presence of the Hoechst dye. The sequence-specific affinity for Hoechst 33258 was calculated as the increase in melting temperature caused by ligand binding. The dye exhibited specificity for A:T but not G:C base pairs. The affinity is low for two A:T base pairs, increases significantly for three, and reaches a plateau for four A:T base pairs. The relative ligand affinity for all trinucleotide and tetranucleotide sequences (A/T)(3)and (A/T)(4)was estimated. The free energy of dye binding to several duplexes was calculated from the equilibrium melting curves of the duplexes formed on the oligonucleotide microchips. This method can be used as a general approach for massive screening of the sequence specificity of DNA-binding compounds.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC); USDOD; FOR
- DOE Contract Number:
- DE-AC02-06CH11357
- OSTI ID:
- 942592
- Report Number(s):
- ANL/BTC/JA-32815; NARHAD; TRN: US200920%%57
- Journal Information:
- Nucleic Acids Res., Vol. 27, Issue 20 ; Oct. 15, 1999; ISSN 0305-1048
- Country of Publication:
- United States
- Language:
- ENGLISH
Similar Records
Binding of Hoechst 33258 and 4 prime ,6-diamidino-2-phenylindole to self-complementary decadeoxynucleotides with modified exocyclic base substituents
Spectroscopic Study of the Binding of Netropsin and Hoechst 33258 to Nucleic Acids