Methods for increasing the production of ethanol from microbial fermentation
- Fayetteville, AR
- Bethlehem, PA
A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.
- Research Organization:
- BIOENGINEERING RESOURCES, INC.
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FC04-94AL98770
- Assignee:
- Emmaus Foundation, Inc. (Fayetteville, AK)
- Patent Number(s):
- 7,285,402
- Application Number:
- 10/311,655
- OSTI ID:
- 941118
- Country of Publication:
- United States
- Language:
- English
Clostridium carboxidivorans sp. nov., a solvent-producing clostridium isolated from an agricultural settling lagoon, and reclassification of the acetogen Clostridium scatologenes strain SL1 as Clostridium drakei sp. nov.
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journal | September 2005 |
Introduction to the Proceedings of the Fourteenth Symposium on Biotechnology for Fuels and Chemicals
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journal | September 1993 |
Biological conversion of coal and coal-derived synthesis gas
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journal | December 1993 |
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