Crystallization and preliminary structure of porcine aldehyde reductase-NADPH binary complex.
Journal Article
·
· Acta Crystallogr. D
Porcine aldehyde reductase-NADPH binary complex has been crystallized from a buffered ammonium sulfate solution. The crystal form is hexagonal, space group P6{sub 5}22, with a = b = 67.2, c = 243.7 A, {alpha} = {beta} = 90.0 and {gamma} = 120.0 degrees. A molecular-replacement structure solution has been successfully obtained by using the refined structure of the apoenzyme as the search model. The crystallographic R-factor is currently equal to 0.24 after energy minimization using data between 8 and 3.0 angstrom resolution. The aldehyde reductase-NADPH complex model is supported by electron density corresponding to NADPH not included in the search model. The tertiary structure of aldehyde reductase consists of a {beta}/{alpha}-barrel with the coenzyme-binding site located at the carboxy-terminal end of the strands of the barrel. The structure of aldehyde reductase-NADPH binary complex will help clarify the mechanism of action for this enzyme and will lead to the development of pharmacologic agents to delay or prevent diabetic complications.
- Research Organization:
- Argonne National Laboratory (ANL)
- DOE Contract Number:
- AC02-06CH11357
- OSTI ID:
- 937877
- Report Number(s):
- ANL/CMB/JA-22600
- Journal Information:
- Acta Crystallogr. D, Journal Name: Acta Crystallogr. D Journal Issue: 1995 Vol. 51
- Country of Publication:
- United States
- Language:
- ENGLISH
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