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Bayesian-Integrated Microbial Forensics

Journal Article · · Applied and Environmental Microbiology

In the aftermath of the 2001 anthrax letters, researchers have been exploring ways to predict the production environment of unknown source microorganisms. Different mass spectral techniques are being developed to characterize components of a microbe’s culture medium including water, carbon and nitrogen sources, metal ions added, and the presence of agar. Individually, each technique has the potential to identify one or two ingredients in a culture medium recipe. However, by integrating data from multiple mass spectral techniques, a more complete characterization is possible. We present a Bayesian statistical approach to integrated microbial forensics and illustrate its application on spores grown in different culture media.

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-76RL01830
OSTI ID:
936755
Report Number(s):
PNNL-SA-59675
Journal Information:
Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 11 Vol. 74; ISSN AEMIDF; ISSN 0099-2240
Country of Publication:
United States
Language:
English

References (21)

The Stability of Messenger Ribonucleic Acid during Sporulation in Bacillus subtilis journal May 1971
Influence of diet on the distribution of nitrogen isotopes in animals journal March 1981
Laser-induced breakdown spectroscopy of bacterial spores, molds, pollens, and protein: initial studies of discrimination potential journal January 2003
Influence of diet on the distribution of carbon isotopes in animals journal May 1978
Quantitative Determination of Heme for Forensic Characterization of Bacillus Spores Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry journal May 2004
Metal speciation and microbial growth--the hard (and soft) facts journal April 1991
Effects of varied pH, growth rate and temperature using controlled fermentation and batch culture on Matrix Assisted Laser Desorption/Ionization whole cell protein fingerprints journal September 2005
Bacterial analysis by MALDI-TOF mass spectrometry: An inter-laboratory comparison journal April 2005
Localization, Annotation, and Comparison of the Escherichia coli K-12 Proteome under Two States of Growth journal May 2005
Development of fine Structure, Thermostability, and Dipicolinate During Sporogenesis in a Bacillus journal April 1960
New guidelines for reporting stable hydrogen, carbon, and oxygen isotope-ratio data journal September 1996
Effect of Culture Conditions on Microorganism Identification by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry journal January 2005
Bayesian Networks and Decision Graphs book January 2001
Stable Isotope Ratios and Forensic Analysis of Microorganisms journal April 2007
Femtomolar Sensitivity of Metalloregulatory Proteins Controlling Zinc Homeostasis journal June 2001
Toward a System of Microbial Forensics: from Sample Collection to Interpretation of Evidence journal May 2005
Forty Years of Bacterial Fatty Acid Synthesis journal April 2002
Microbe forensics: Oxygen and hydrogen stable isotope ratios in Bacillus subtilis cells and spores journal January 2003
Differentiation of Spores of Bacillus subtilis Grown in Different Media by Elemental Characterization Using Time-of-Flight Secondary Ion Mass Spectrometry journal November 2005
The Phosphorus Source Phytate Changes the Composition of the Cell Wall Proteome in B acillus subtilis journal January 2007
Genetic Analysis and Attribution of Microbial Forensics Evidence journal January 2005

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