Determining physical constraints in transcriptional initiationcomplexes using DNA sequence analysis
Journal Article
·
· Public Library of Science
OSTI ID:932488
Eukaryotic gene expression is often under the control ofcooperatively acting transcription factors whose binding is limited bystructural constraints. By determining these structural constraints, wecan understand the "rules" that define functional cooperativity.Conversely, by understanding the rules of binding, we can inferstructural characteristics. We have developed an information theory basedmethod for approximating the physical limitations of cooperativeinteractions by comparing sequence analysis to microarray expressiondata. When applied to the coordinated binding of the sulfur amino acidregulatory protein Met4 by Cbf1 and Met31, we were able to create acombinatorial model that can correctly identify Met4 regulatedgenes.
- Research Organization:
- COLLABORATION - UCBerkeley
- Sponsoring Organization:
- USDOE Director, Office of Science; National Institutes ofHealth
- DOE Contract Number:
- AC02-05CH11231
- OSTI ID:
- 932488
- Report Number(s):
- LBNL--63065; BnR: 400412000
- Journal Information:
- Public Library of Science, Journal Name: Public Library of Science Journal Issue: 11 Vol. 2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Determining Physical Constraints in Transcriptional Initiation Complexes Using DNA Sequence Analysis
Flexible promoter architecture requirements for coactivator recruitment
Journal Article
·
Tue Nov 20 19:00:00 EST 2007
· PLoS ONE
·
OSTI ID:1627339
Flexible promoter architecture requirements for coactivator recruitment
Journal Article
·
Thu Apr 27 20:00:00 EDT 2006
· BMC Molecular Biology
·
OSTI ID:1626499