A microbial fuel cell operating at low pH using an acidophile, Acidiphilium cryptum.

Journal Article · Tue Jan 01 04:00:00 EST 2008 · Biotechnology Letters

DOI:https://doi.org/10.1007/s10529-008-9700-y· OSTI ID:931108

Borole, Abhijeet P ^[1]; Cesar, Scott A ^[1]; O'Neill, Hugh Michael ^[1]; Tsouris, Costas ^[1]

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1. ORNL

A microbial fuel cell using an acidophilic microorganism, Acidiphilium cryptum, as the anode biocatalyst was investigated. The mode of electron transfer by this organism to the electrode was studied. Electricity production in the presence of a mediator was demonstrated using its natural electron acceptor, iron, as well as phenosafranin as the electron mediating agent. Production of Fe(II), as a result of iron reduction, at a pH of 4.0 or below was found to support electricity production. Accumulation of the oxidized iron, Fe(III) as a result of electron donation to the electrode, however, restricted higher current output. Addition of nitrilotriacetic acid helped resolve the problem by redissolution of deposited Fe(III). Further, use of phenosafranin as a secondary mediator resulted in improvement in power output. At a cell loading equivalent to OD600 of 1.0, a power output of 12.7 mW/m2 was obtained in a two-chamber air-sparged fuel cell. Potential for direct electron transfer was also investigated but not detected under the conditions studied.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: ORNL LDRD Director's R&D
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 931108
• Journal Information: Biotechnology Letters, Journal Name: Biotechnology Letters Journal Issue: 8 Vol. 30; ISSN 0141-5492
• Country of Publication: United States
• Language: English

Related Subjects

Acidiphilium cryptum
acidophile
biofuel cell
electricity
mediated electron transfer