Liquid Microjunction Surface Sampling Probe Electrospray Mass Spectrometry for Detection of Drugs and Metabolites in Thin Tissue Sections

Journal Article · Tue Jan 01 04:00:00 EST 2008 · Journal of Mass Spectrometry

DOI:https://doi.org/10.1002/jms.1340· OSTI ID:931020

Van Berkel, Gary J ^[1]; Kertesz, Vilmos ^[1]; Koeplinger, Kenneth A. ^[2]; Vavek, Marissa ^[2]; Kong, Ah-Ng Tony ^[3]

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1. ORNL
2. Merck Research Laboratories
3. Rutgers University

A self-aspirating, liquid micro-junction surface sampling probe/electrospray emitter mass spectrometry system was demonstrated for use in the direct analysis of spotted and dosed drugs and their metabolites in thin tissue sections. Proof-of-principle sampling and analysis directly from tissue without the need for sample preparation was demonstrated first by raster scanning a region on a section of rat liver onto which reserpine was spotted. The mass spectral signal from selected reaction monitoring was used to develop a chemical image of the spotted drug on the tissue. The probe was also used to selectively spot sample areas of sagittal whole mouse body tissue sections that had been dosed orally (90 mg/kg) with R,S-sulforaphane 3 hrs prior to sacrifice. Sulforaphane and its glutathione and N-acetyl cysteine conjugates were monitored with selected reaction monitoring and detected in the stomach and various other tissues from the dosed mouse. No signal for these species was observed in the tissue from a control mouse. The same dosed tissue section was used to illustrate the possibility of obtaining a line scan across the whole body section. In total these results illustrate the potential for rapid screening of the distribution of drugs and metabolites in tissue sections with the micro-liquid junction surface sampling probe/electrospray mass spectrometry approach.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: ORNL work for others
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 931020
• Journal Information: Journal of Mass Spectrometry, Journal Name: Journal of Mass Spectrometry Journal Issue: 4 Vol. 43; ISSN 1076-5174
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
CYSTEINE
DETECTION
DRUGS
GLUTATHIONE
LIVER
MASS SPECTROSCOPY
METABOLITES
MICE
RATS
RESERPINE
SPRAYS
STOMACH
electrospray ionization
mass spectrometry
metabolites
reserpine
sulforaphane
surface sampling probe
tissue section