Synthesis of a 2'-Se-thymidine Phosphoramidite and Its Incorporation into Oligonucleotides for Crystal Structure Study
To investigate nucleic acids with selenium derivatization for crystallography, we report the first synthesis of 2'-methylseleno-thymidine phosphoramidite and its incorporation into DNAs and RNAs by solid-phase synthesis with over 99% coupling yield. The d(GT{sub Se}GTACAC){sub 2} crystal structure was also determined at 1.40 {angstrom} resolution using Se phasing, revealing that this Se derivatization did not cause significant structure perturbation, consistent with our UV melting study. In addition, we observed that the Se modification largely facilitated the crystallization.
- Research Organization:
- Brookhaven National Laboratory (BNL) National Synchrotron Light Source
- Sponsoring Organization:
- Doe - Office Of Science
- DOE Contract Number:
- AC02-98CH10886
- OSTI ID:
- 930319
- Report Number(s):
- BNL--81029-2008-JA
- Journal Information:
- Organic Letters, Journal Name: Organic Letters Journal Issue: 5 Vol. 9; ISSN 1523-7060
- Country of Publication:
- United States
- Language:
- English
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