Structural Basis for Ubiquitin Recognition by the Human ESCRT-II EAP45 GLUE Domain

Journal Article · Sun Jan 01 00:00:00 EST 2006 · Nature Structural and Molecular Biology

DOI:https://doi.org/10.1038/nsmb1160· OSTI ID:930210

Alam, S; Langelier, C; Whitby, F; Koirala, S; Robinson, H; Hill, C; Sundquist, W

ESCRT-IESCRT-IIGLUEEAP45VPS36The ESCRT-I and ESCRT-II complexes help sort ubiquitinated proteins into vesicles that accumulate within multivesicular bodies (MVBs). Crystallographic and biochemical analyses reveal that the GLUE domain of the human ESCRT-II EAP45 (also called VPS36) subunit is a split pleckstrin-homology domain that binds ubiquitin along one edge of the {beta}-sandwich. The structure suggests how human ESCRT-II can couple recognition of ubiquitinated cargoes and endosomal phospholipids during MVB protein sorting.

• Research Organization: Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source
• Sponsoring Organization: Doe - Office Of Science
• DOE Contract Number: DE-AC02-98CH10886
• OSTI ID: 930210
• Report Number(s): BNL-80873-2008-JA; TRN: US200822%%1389
• Journal Information: Nature Structural and Molecular Biology, Vol. 13, Issue 11
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
COMPLEXES
HUMAN POPULATIONS
PHOSPHOLIPIDS
PROTEINS
SORTING
national synchrotron light source