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Title: Crystallization and Preliminary X-ray Diffraction Analysis of Salmonella typhi PilS

Abstract

The structure determination of PilS, a type IV pilin, by X-ray crystallography is reported. The recombinant protein from Salmonella typhi was overexpressed, purified and crystallized. The crystals belong to space group P2{sub 1}2{sub 1}2, with unit-cell parameters a = 77.88, b = 114.53, c = 31.75 {angstrom}. The selenomethionine derivative of the PilS protein was overexpressed, purified and crystallized in the same space group. Data sets have been collected to 2.1 {angstrom} resolution from the selenomethionine-derivative crystal using synchrotron radiation for multiwavelength anomalous dispersion (MAD) phasing.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL) National Synchrotron Light Source
Sponsoring Org.:
Doe - Office Of Science
OSTI Identifier:
930092
Report Number(s):
BNL-80728-2008-JA
TRN: US0806702
DOE Contract Number:
DE-AC02-98CH10886
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica Section F: Structural Biology and Crystallization Communications; Journal Volume: 62
Country of Publication:
United States
Language:
English
Subject:
43 PARTICLE ACCELERATORS; CRYSTALLIZATION; CRYSTALLOGRAPHY; CRYSTALS; DATA; DISPERSIONS; PROTEINS; RESOLUTION; SALMONELLA; SPACE GROUPS; SYNCHROTRON RADIATION; X-RAY DIFFRACTION; national synchrotron light source

Citation Formats

Balakrishna,A., Tan, Y., Mok, H., Saxena, A., and Swaminathan, K. Crystallization and Preliminary X-ray Diffraction Analysis of Salmonella typhi PilS. United States: N. p., 2006. Web. doi:10.1107/S174430910603661X.
Balakrishna,A., Tan, Y., Mok, H., Saxena, A., & Swaminathan, K. Crystallization and Preliminary X-ray Diffraction Analysis of Salmonella typhi PilS. United States. doi:10.1107/S174430910603661X.
Balakrishna,A., Tan, Y., Mok, H., Saxena, A., and Swaminathan, K. Sun . "Crystallization and Preliminary X-ray Diffraction Analysis of Salmonella typhi PilS". United States. doi:10.1107/S174430910603661X.
@article{osti_930092,
title = {Crystallization and Preliminary X-ray Diffraction Analysis of Salmonella typhi PilS},
author = {Balakrishna,A. and Tan, Y. and Mok, H. and Saxena, A. and Swaminathan, K.},
abstractNote = {The structure determination of PilS, a type IV pilin, by X-ray crystallography is reported. The recombinant protein from Salmonella typhi was overexpressed, purified and crystallized. The crystals belong to space group P2{sub 1}2{sub 1}2, with unit-cell parameters a = 77.88, b = 114.53, c = 31.75 {angstrom}. The selenomethionine derivative of the PilS protein was overexpressed, purified and crystallized in the same space group. Data sets have been collected to 2.1 {angstrom} resolution from the selenomethionine-derivative crystal using synchrotron radiation for multiwavelength anomalous dispersion (MAD) phasing.},
doi = {10.1107/S174430910603661X},
journal = {Acta Crystallographica Section F: Structural Biology and Crystallization Communications},
number = ,
volume = 62,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2006},
month = {Sun Jan 01 00:00:00 EST 2006}
}
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