Global Structure Changes Associated with Ca2+ Activation of Full-length Human Plasma Gelsolin

Journal Article · Mon Jan 01 04:00:00 EST 2007 · Journal of Biological Chemistry

DOI:https://doi.org/10.1074/jbc.M702446200· OSTI ID:930001

Ashish, F; Paine, M; Perryman, P; Yang, L; Yin, H; Krueger, J

Gelsolin regulates the dynamic assembly and disassembly of the actin-based cytoskeleton in non-muscle cells and clears the circulation of filaments released following cell death. Gelsolin is a six-domain (G1-G6) protein activated by calcium via a multi-step process that involves unfolding from a compact form to a more open form in which the three actin-binding sites (on the G1, G2, and G4 subdomains) become exposed. To follow the global structural changes that accompany calcium activation of gelsolin, small-angle x-ray scattering (SAXS) data were collected for full-length human plasma gelsolin at nanomolar to millimolar concentrations of free Ca{sup 2+}. Analysis of these data showed that, upon increasing free Ca{sup 2+} levels, the radius of gyration (R{sub g}) increased nearly 12 {angstrom}, from 31.1 {+-} 0.3 to 43 {+-} 2 {angstrom}, and the maximum linear dimension (D{sub max}) of the gelsolin molecule increased 55 {angstrom}, from 100 to 155{angstrom}. Structural reconstruction of gelsolin from these data provided a striking visual tracking of the gradual Ca{sup 2+}-induced opening of the gelsolin molecule and highlighted the critical role played by the flexible linkers between homologous domains. The tightly packed architecture of calcium-free gelsolin, seen from both SAXS and x-ray crystallographic models, is already partially opened up in as low as 0.5 nM Ca{sup 2+}. Our data confirm that, although the molecule springs open from 0 to 1 {mu} free Ca{sup 2+}, even higher calcium concentrations help to stabilize a more open structure, with increases in R{sub g} and D{sub max} of 2 and 15 {angstrom}, respectively. At these higher calcium levels, the SAXS-based models provide a molecular shape that is compatible with that of the crystal structures solved for Ca{sup 2+}/gelsolin C-terminal and N-terminal halves {+-} monomeric G-actin. Placement of these crystal structures within the boundaries of the SAXS-based model suggests a movement of the G1/G2 subunits that would be required upon binding to actin.

• Research Organization: Brookhaven National Laboratory (BNL) National Synchrotron Light Source
• Sponsoring Organization: Doe - Office Of Science
• DOE Contract Number: AC02-98CH10886
• OSTI ID: 930001
• Report Number(s): BNL--80612-2008-JA
• Journal Information: Journal of Biological Chemistry, Journal Name: Journal of Biological Chemistry Journal Issue: 35 Vol. 282; ISSN JBCHA3; ISSN 0021-9258
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE
ACTIN
ANIMAL CELLS
AUGMENTATION
CALCIUM
CRYSTAL STRUCTURE
DEATH
FILAMENTS
HUMAN POPULATIONS
LEVELS
MOLECULES
PLASMA
PROTEINS
SCATTERING
SHAPE
SMALL ANGLE SCATTERING
X-RAY DIFFRACTION
national synchrotron light source