Gel Electrophoresis of Gold-DNA Nano-Conjugates
Single stranded DNA of different lengths and different amounts was attached to colloidal phosphine stabilized Au nanoparticles. The resulting conjugates were investigated in detail by a gel electrophoresis study based on 1200 gels. We demonstrate how these experiments help to understand the binding of DNA to Au particles. In particular we compare specific attachment of DNA via gold-thiol bonds with nonspecific adsorption of DNA. The maximum number of DNA molecules that can be bound per particle was determined. We also compare several methods to used gel electrophoresis for investigating the effective diameter of DNA-Au conjugates, such as using a calibration curve of particles with known diameters and Ferguson plots.
- Research Organization:
- COLLABORATION - U.Muenchen/Germany
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 929759
- Report Number(s):
- LBNL-61541; R&D Project: 516070; BnR: KC0203010; TRN: US200812%%650
- Journal Information:
- Journal of Biomedicine and Biotechnology, Vol. 2007, Issue 10; Related Information: Journal Publication Date: 2007
- Country of Publication:
- United States
- Language:
- English
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