Genome-wide experimental determination of barriers to horizontal gene transfer

Journal Article · Mon Sep 24 04:00:00 EDT 2007 · Science

OSTI ID:927148

Rubin, Edward; Sorek, Rotem; Zhu, Yiwen; Creevey, Christopher J; Francino, M Pilar; Bork, Peer; Rubin, Edward M

Horizontal gene transfer, in which genetic material is transferred from the genome of one organism to another, has been investigated in microbial species mainly through computational sequence analyses. To address the lack of experimental data, we studied the attempted movement of 246,045 genes from 79 prokaryotic genomes into E. coli and identified genes that consistently fail to transfer. We studied the mechanisms underlying transfer inhibition by placing coding regions from different species under the control of inducible promoters. Their toxicity to the host inhibited transfer regardless of the species of origin and our data suggest that increased gene dosage and associated increased expression is a predominant cause for transfer failure. While these experimental studies examined transfer solely into E. coli, a computational analysis of gene transfer rates across available bacterial and archaeal genomes indicates that the barriers observed in our study are general across the tree of life.

• Research Organization: Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
• Sponsoring Organization: Genomics Division
• DOE Contract Number: AC02-05CH11231
• OSTI ID: 927148
• Report Number(s): LBNL-157E
• Journal Information: Science, Journal Name: Science Journal Issue: 5855 Vol. 318; ISSN 0193-4511; ISSN SCEHDK
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
GENES
GENETICS
Genome gene transfer DNA
ORIGIN
PROMOTERS
TOXICITY
TREES