Cellular Response of Shewanella oneidensis to StrontiumStress
Journal Article
·
· Applied and Environmental Microbiology
OSTI ID:922807
The physiology and transcriptome dynamics of the metalion-reducing bacterium Shewanella oneidensis strain MR-1 in response tononradioactive strontium (Sr) exposure were investigated. Studiesindicated that MR-1 was able to grow aerobically in complex medium in thepresence of 180 mMSrCl2 but showed severe growth inhibition at levelsabove that concentration. Temporal gene expression profiles weregenerated from aerobically grown, mid-exponential-phase MR-1 cellsshocked with 180 mM SrCl2 and analyzed for significant differences inmRNA abundance with reference to data for nonstressed MR-1 cells. Geneswith annotated functions in siderophore biosynthesis and iron transportwere among the most highly induced (>100-fold [P<0.05]) openreading frames in response to acute Sr stress, and a mutant(SO3032::pKNOCK) defective in siderophore production was found to behypersensitive to SrCl2 exposure, compared to parental and wild-typestrains. Transcripts encoding multidrug and heavy metal efflux pumps,proteins involved in osmotic adaptation, sulfate ABC transporters, andassimilative sulfur metabolism enzymes also were differentially expressedfollowing Sr exposure but at levels that were several orders of magnitudelower than those for iron transport genes. Precipitate formation wasobserved during aerobic growth of MR-1 in broth cultures amended with 50,100, or 150 mM SrCl2 but not in cultures of the SO3032::pKNOCK mutant orin the abiotic control. Chemical analysis of this precipitate usinglaser-induced breakdown spectroscopy and static secondary ion massspectrometry indicated extracellular solid-phase sequestration of Sr,with at least a portion of the heavy metal associated with carbonatephases.
- Research Organization:
- COLLABORATION - ORNL
- DOE Contract Number:
- AC02-05CH11231
- OSTI ID:
- 922807
- Report Number(s):
- LBNL--60417; BnR: KP1102010
- Journal Information:
- Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 1 Vol. 72; ISSN AEMIDF; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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