Fully automated four-column capillary LC-MS system for maximizing throughput in proteomic analyses

Journal Article · Tue Jan 01 04:00:00 EST 2008 · Analytical Chemistry, 80(1):294-302

DOI:https://doi.org/10.1021/ac701727r· OSTI ID:921809

Livesay, Eric A; Tang, Keqi; Taylor, Beverley K; Buschbach, Michael A; Hopkins, Derek F; Lamarche, Brian L; Zhao, Rui; Shen, Yufeng; Orton, Daniel J; Moore, Ronald J; Kelly, Ryan T; Udseth, Harold R; Smith, Richard D

We describe a 4-column, high-pressure capillary liquid chromatography (LC) system for robust, high-throughput LC-MS(/MS) analyses. This system performs multiple LC separations in parallel, but staggers each of them such that the data-rich region of each separation is sampled sequentially. By allowing nearly continuous data acquisition, this design maximizes the use of the mass spectrometer. Each analytical column is connected to a corresponding ESI emitter in order to avoid the use of post-column switching and associated dead volume issues. Encoding translation stages are employed to sequentially position the emitters at the MS inlet. The high reproducibility of this system is demonstrated using consecutive analyses of global tryptic digest of the microbe Shewanella oneidensis.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 921809
• Report Number(s): PNNL-SA-56532; 400412000
• Journal Information: Analytical Chemistry, 80(1):294-302, Journal Name: Analytical Chemistry, 80(1):294-302 Journal Issue: 1 Vol. 80
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
AUTOMATION
CAPILLARY FLOW
DATA ACQUISITION
DESIGN
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROMETERS