Flow Through PCR Module of BioBriefcase

Conference · Mon Sep 19 00:00:00 EDT 2005

DOI:https://doi.org/10.1117/12.630844· OSTI ID:919211

Arroyo, E S; Wheeler, E K; Hindson, B; Nasarabadi, S; Vrankovich, G; Bell, P; Bailey, C; Sheppod, T; Christian, A

The BioBriefcase is an integrated briefcase-sized aerosol collection and analysis system for autonomous monitoring of the environment, which is currently being jointly developed by Lawrence Livermore and Sandia National Laboratories. This poster presents results from the polymerase chain reaction (PCR) module of the system. The DNA must be purified after exiting the aerosol collector to prevent inhibition of the enzymatic reaction. Traditional solid-phase extraction results in a large loss of sample. In this flow-through system, we perform sample purification, concentration and amplification in one reactor, which minimizes the loss of material. The sample from the aerosol collector is mixed with a denaturation solution prior to flowing through a capillary packed with silica beads. The DNA adheres to the silica beads allowing the environmental contaminants to be flushed to waste while effectively concentrating the DNA on the silica matrix. The adhered DNA is amplified while on the surface of the silica beads, resulting in a lower limit of detection than an equivalent eluted sample. Thus, this system is beneficial since more DNA is available for amplification, less reagents are utilized, and contamination risks are reduced.

• Research Organization: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 919211
• Report Number(s): UCRL-PROC-215517; TRN: US200822%%165
• Resource Relation: Journal Volume: 6007; Conference: Presented at: Optics East, Boston, MA, United States, Oct 23 - Oct 26, 2005
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
71 CLASSICAL AND QUANTUMM MECHANICS
GENERAL PHYSICS
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
47 OTHER INSTRUMENTATION
AEROSOLS
AMPLIFICATION
CONTAMINATION
DETECTION
DNA
MONITORING
OPTICS
POLYMERASE CHAIN REACTION
PURIFICATION
SANDIA NATIONAL LABORATORIES
SILICA
WASTES