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Enzymatic cascade bioreactor

Patent ·
OSTI ID:914637
 [1];  [2];  [3];  [4]
  1. San Francisco, CA
  2. Livermore, CA
  3. Albuquerque, NM
  4. Woodinville, WA
+ Show Author Affiliations
Disclosed is an apparatus and method for continuously converting sucrose to .beta.-D-glucose. The method comprises a three stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and .alpha.-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output.
Research Organization:
Sandia National Laboratories (SNL-CA), Livermore, CA
Sponsoring Organization:
United States Department of Energy
DOE Contract Number:
AC04-94AL85000
Assignee:
Sandia Corporation (Livermore, CA)
Patent Number(s):
7,264,962
Application Number:
11/080,901
OSTI ID:
914637
Country of Publication:
United States
Language:
English

References (10)

Trienzymatic biosensor for the determination of inorganic phosphate journal September 2001
Enzymatic Conversion of Sucrose to Hydrogen journal December 1998
A Miniature Biofuel Cell journal September 2001
A Miniature Biofuel Cell Operating in A Physiological Buffer journal November 2002
A Three-Cascaded-Enzymes Biosensor to Determine Lactose Concentration in Raw Milk journal September 2000
A Miniature Membrane-less Biofuel Cell Operating under Physiological Conditions at 0.5 V journal January 2003
Enhancement of operational stability of an enzyme biosensor for glucose and sucrose using protein based stabilizing agents journal June 2002
Optimization of the multienzyme system for sucrose biosensor by response surface methodology journal August 2001
Rapid determination of glucose and sucrose by an amperometric glucose-sensing electrode combined with an invertase/mutarotase-attached measuring cell journal November 1997
Miniature biofuel cells journal January 2004

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59 BASIC BIOLOGICAL SCIENCES