Catching RNA Polymerase in the act of Binding: Intermediates in Transcription Illuminated by Synchrotron Footprinting

Journal Article · Fri Dec 31 23:00:00 EST 2004 · Proc Natl Acad Sci USA

DOI:https://doi.org/10.1073/pnas.0501152102· OSTI ID:913907

Brenowitz, M; Erie, D; Chance, M

The article by Sclavi et al. in this issue of PNAS addresses 'initiation, ' the first step in transcription. Gene transcription is catalyzed in cells by large multisubunit proteins called RNA polymerases (RNAP). The eubacteria holoenzyme of RNAP is composed of five core subunits ({alpha}, {alpha}2, {beta}, {beta}', and {omega}) that contain the amino acid residues required for the enzyme's catalytic activity. A sixth subunit ({sigma}) guides RNAP to specific sequences on the genomic DNA (promoters) that mark the beginning of a gene or group of genes.

• Research Organization: Brookhaven National Laboratory (BNL) National Synchrotron Light Source
• Sponsoring Organization: Doe - Office Of Science
• DOE Contract Number: AC02-98CH10886
• OSTI ID: 913907
• Report Number(s): BNL--78475-2007-JA
• Journal Information: Proc Natl Acad Sci USA, Journal Name: Proc Natl Acad Sci USA Vol. 102; ISSN 0027-8424; ISSN PNASA6
• Country of Publication: United States
• Language: English

Related Subjects

43 PARTICLE ACCELERATORS
AMINO ACIDS
DNA
GENES
PROMOTERS
PROTEINS
RESIDUES
RNA POLYMERASES
SYNCHROTRONS
TRANSCRIPTION
national synchrotron light source