Catching RNA Polymerase in the act of Binding: Intermediates in Transcription Illuminated by Synchrotron Footprinting
Journal Article
·
· Proc Natl Acad Sci USA
The article by Sclavi et al. in this issue of PNAS addresses 'initiation, ' the first step in transcription. Gene transcription is catalyzed in cells by large multisubunit proteins called RNA polymerases (RNAP). The eubacteria holoenzyme of RNAP is composed of five core subunits ({alpha}, {alpha}2, {beta}, {beta}', and {omega}) that contain the amino acid residues required for the enzyme's catalytic activity. A sixth subunit ({sigma}) guides RNAP to specific sequences on the genomic DNA (promoters) that mark the beginning of a gene or group of genes.
- Research Organization:
- Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source
- Sponsoring Organization:
- Doe - Office Of Science
- DOE Contract Number:
- DE-AC02-98CH10886
- OSTI ID:
- 913907
- Report Number(s):
- BNL-78475-2007-JA; PNASA6; TRN: US0801400
- Journal Information:
- Proc Natl Acad Sci USA, Vol. 102; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
Similar Records
Transcription inactivation through local refolding of the RNA polymerase structure
Structural basis of template strand deoxyuridine promoter recognition by a viral RNA polymerase
Recombinant Thermus aquaticus RNA Polymerase for Structural Studies
Journal Article
·
Thu Feb 12 00:00:00 EST 2009
· Nature
·
OSTI ID:913907
+8 more
Structural basis of template strand deoxyuridine promoter recognition by a viral RNA polymerase
Journal Article
·
Mon Jun 20 00:00:00 EDT 2022
· Nature Communications
·
OSTI ID:913907
+5 more
Recombinant Thermus aquaticus RNA Polymerase for Structural Studies
Journal Article
·
Sun Jan 01 00:00:00 EST 2006
· J. Mol. Biol.
·
OSTI ID:913907
+3 more