Crystal Structure of the Protein Kinase Domain of Yeast AMP-Activated Protein Kinase Snf1
Journal Article
·
· Biochem. Biophys. Res. Commun.
AMP-activated protein kinase (AMPK) is a master metabolic regulator, and is an important target for drug development against diabetes, obesity, and other diseases. AMPK is a hetero-trimeric enzyme, with a catalytic ({alpha}) subunit, and two regulatory ({beta} and {gamma}) subunits. Here we report the crystal structure at 2.2 Angstrom resolution of the protein kinase domain (KD) of the catalytic subunit of yeast AMPK (commonly known as SNF1). The Snf1-KD structure shares strong similarity to other protein kinases, with a small N-terminal lobe and a large C-terminal lobe. Two negative surface patches in the structure may be important for the recognition of the substrates of this kinase.
- Research Organization:
- Brookhaven National Laboratory (BNL) National Synchrotron Light Source
- Sponsoring Organization:
- Doe - Office Of Science
- DOE Contract Number:
- AC02-98CH10886
- OSTI ID:
- 913706
- Report Number(s):
- BNL--78274-2007-JA
- Journal Information:
- Biochem. Biophys. Res. Commun., Journal Name: Biochem. Biophys. Res. Commun. Vol. 337; ISSN BBRCA9; ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
Similar Records
An Inhibited Conformation for the Protein Kinase Domain of the Saccharomyces cerevisiae AMPK Homolog Snf1
Crystallization of the glycogen-binding domain of the AMP-activated protein kinase β subunit and preliminary X-ray analysis
Crystal Structure of the Heterotrimer Core of Saccharomyces cerevisiae AMPK Homologue SNF1
Journal Article
·
Thu Dec 31 23:00:00 EST 2009
· Acta Crystallographica Section F: Structural Biology and Crystallization Communications
·
OSTI ID:1020106
Crystallization of the glycogen-binding domain of the AMP-activated protein kinase β subunit and preliminary X-ray analysis
Journal Article
·
Fri Dec 31 23:00:00 EST 2004
· Acta Crystallographica. Section F
·
OSTI ID:22356070
Crystal Structure of the Heterotrimer Core of Saccharomyces cerevisiae AMPK Homologue SNF1
Journal Article
·
Sun Dec 31 23:00:00 EST 2006
· Nature
·
OSTI ID:959509