Genetic study of the pVM82 plasmid responsible for some pathogenic traits of Yersinia pseudotuberculosis
- Gamaleya Institute of Epidemiology and Microbiology, Moscow (Russian Federation)
The large pVM82 plasmid isolated epidemic strains of Yersinia pseudotuberculosis includes the 25 MDa segment, which encodes a series of properties affecting the virulence of the bacterium. Insertion mutants of pVM82 containing transposition-defective Tn2507 with a kanamycin-resistance marker in different Hind III fragments of the 25 MDa segment were obtained. By recombination between two homologous pVM82 containing genetic markers in different parts, deletion derivatives of pVM82 plasmid and insertions of the plasmid segment, carrying a kanamycin-resistance marker, into a chromosome were obtained. Results were obtained suggesting the presence in the plasmid 25 MDa segment of a transposon-like structure capable of migrating from pVM82 plasmid onto a chromosome and from a chromosome and pVM82 onto pRP1.2 plasmid of a broad host range. 8 refs., 4 figs., 1 tab.
- OSTI ID:
- 91046
- Journal Information:
- Russian Journal of Genetics, Journal Name: Russian Journal of Genetics Journal Issue: 7 Vol. 31; ISSN RJGEEQ; ISSN 1022-7954
- Country of Publication:
- United States
- Language:
- English
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