Glycoprotein synthesis

Shultz, Peter G; Wang, Lei; Zhang, Zhiwen

Title: Glycoprotein synthesis

Abstract

Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.

Inventors:

Shultz, Peter G ^[1]; Wang, Lei ^[2]; Zhang, Zhiwen ^[2]

La Jolla, CA
San Diego, CA

Publication Date:: 2007-04-03

Research Org.:: The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 907753

Patent Number(s):: 7,199,222

Application Number:: 11/093,597

Assignee:: The Scripps Research Institute (La Jolla, CA)

DOE Contract Number:: FG03-00ER45812

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Shultz, Peter G, Wang, Lei, and Zhang, Zhiwen. Glycoprotein synthesis.  United States: N. p., 2007. 
        Web.

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                    Shultz, Peter G, Wang, Lei, & Zhang, Zhiwen. Glycoprotein synthesis.  United States.

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                    Shultz, Peter G, Wang, Lei, and Zhang, Zhiwen. 2007.  
        "Glycoprotein synthesis".  United States.  https://www.osti.gov/servlets/purl/907753.

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@article{osti_907753,

  title        = {Glycoprotein synthesis},

  author       = {Shultz, Peter G and Wang, Lei and Zhang, Zhiwen},

  abstractNote = {Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/907753},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2007},

  month        = {4}

}

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Glycoprotein synthesis

Patent Schultz, Peter; Wang, Lei; Zhang, Zhiwen

Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.
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Glycoprotein synthesis

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Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.
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Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.
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Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.
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Similar Records

Title: Glycoprotein synthesis

Abstract

Citation Formats

Dynamic Glycosylation of the Transcription Factor CREB: A Potential Role in Gene Regulation journal, June 2003

Progress toward the evolution of an organism with an expanded genetic code journal, April 1999

Glycosylation of Nucleocytoplasmic Proteins: Signal Transduction and O-GlcNAc journal, March 2001

Engineering Chemical Reactivity on Cell Surfaces Through Oligosaccharide Biosynthesis journal, May 1997

Stereoselective phase transfer catalyzed syntheses of glycosyloxysuccinimides and their transformations into glycoprobes journal, June 1995

Fmoc-Based Synthesis of Peptide- α Thioesters: Application to the Total Chemical Synthesis of a Glycoprotein by Native Chemical Ligation journal, December 1999

A novel method for the specific glycosylation of proteins journal, November 1991

Solution- and Solid-Phase Synthesis of N-Protected Glycopeptide Esters of the Benzyl Type as Substrates for Subtilisin-Catalyzed Glycopeptide Couplings journal, March 1998

Exploring the Limits of Codon and Anticodon Size journal, February 2002

Structurally Modified Firefly Luciferase. Effects of Amino Acid Substitution at Position 286 journal, November 1997

Toward Automated Synthesis of Oligosaccharides and Glycoproteins journal, March 2001

Chemoselective Approaches to Glycoprotein Assembly journal, September 2001

Novel cofactors via post-translational modifications of enzyme active sites journal, July 2000

Chemical Glycobiology journal, March 2001

Enzymatic Glycoprotein Synthesis: Preparation of Ribonuclease Glycoforms via Enzymatic Glycopeptide Condensation and Glycosylation journal, March 1997

Site-Specific Protein Modification Using a Ketone Handle journal, January 1996

Solid-Phase Synthesis of Thioether-Linked Glycopeptide Mimics for Application to Glycoprotein Semisynthesis journal, May 2002

Biodegradation of Aromatic Compounds by Escherichia coli journal, December 2001

Expressed protein ligation: A general method for protein engineering journal, June 1998

Site-directed conjugation of nonpeptide groups to peptides and proteins via periodate oxidation of a 2-amino alcohol. Application to modification at N-terminal serine journal, March 1992

Addition of p-Azido-L-phenylalanine to the Genetic Code of Escherichia coli journal, July 2002

An Expanded Eukaryotic Genetic Code journal, August 2003

A New Strategy for the Site-Specific Modification of Proteins in Vivo journal, June 2003

Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli journal, August 2002

A general approach for the generation of orthogonal tRNAs journal, September 2001

Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli journal, March 2001

An efficient system for the evolution of aminoacyl-tRNA synthetase specificity journal, September 2002

Synthesis of Glycoproteins journal, February 2002

A New Functional Suppressor tRNA/Aminoacyl−tRNA Synthetase Pair for the in Vivo Incorporation of Unnatural Amino Acids into Proteins journal, May 2000

N-Linked Glycosylation in Campylobacter jejuni and Its Functional Transfer into E. coli journal, November 2002

Recent Progress in the Solid-phase Synthesis of Glycopeptide journal, July 2000

Adding l-3-(2-Naphthyl)alanine to the Genetic Code of E. coli journal, March 2002

Biological roles of oligosaccharides: all of the theories are correct journal, April 1993

Intein-Mediated Synthesis of Proteins Containing Carbohydrates and Other Molecular Probes journal, June 2000

Purification and properties of β-mannosyltransferase that synthesizes Man-β-GlcNAc-GlcNAc-pyrophosphoryl-dolichol journal, October 1986

Addition of the keto functional group to the genetic code of Escherichia coli journal, December 2002

Aminooxy-, Hydrazide-, and Thiosemicarbazide-Functionalized Saccharides: Versatile Reagents for Glycoconjugate Synthesis journal, October 1998

Unnatural amino acids as probes of protein structure and function journal, December 2000

Expanding the Genetic Code of Escherichia coli journal, April 2001

Dynamic Glycosylation of the Transcription Factor CREB: A Potential Role in Gene Regulation
journal, June 2003

Progress toward the evolution of an organism with an expanded genetic code
journal, April 1999

Glycosylation of Nucleocytoplasmic Proteins: Signal Transduction and O-GlcNAc
journal, March 2001

Engineering Chemical Reactivity on Cell Surfaces Through Oligosaccharide Biosynthesis
journal, May 1997

Stereoselective phase transfer catalyzed syntheses of glycosyloxysuccinimides and their transformations into glycoprobes
journal, June 1995

Fmoc-Based Synthesis of Peptide- ^α Thioesters: Application to the Total Chemical Synthesis of a Glycoprotein by Native Chemical Ligation
journal, December 1999

A novel method for the specific glycosylation of proteins
journal, November 1991

Solution- and Solid-Phase Synthesis of N-Protected Glycopeptide Esters of the Benzyl Type as Substrates for Subtilisin-Catalyzed Glycopeptide Couplings
journal, March 1998

Exploring the Limits of Codon and Anticodon Size
journal, February 2002

Structurally Modified Firefly Luciferase. Effects of Amino Acid Substitution at Position 286
journal, November 1997

Toward Automated Synthesis of Oligosaccharides and Glycoproteins
journal, March 2001

Chemoselective Approaches to Glycoprotein Assembly
journal, September 2001

Novel cofactors via post-translational modifications of enzyme active sites
journal, July 2000

Chemical Glycobiology
journal, March 2001

Enzymatic Glycoprotein Synthesis: Preparation of Ribonuclease Glycoforms via Enzymatic Glycopeptide Condensation and Glycosylation
journal, March 1997

Site-Specific Protein Modification Using a Ketone Handle
journal, January 1996

Solid-Phase Synthesis of Thioether-Linked Glycopeptide Mimics for Application to Glycoprotein Semisynthesis
journal, May 2002

Biodegradation of Aromatic Compounds by Escherichia coli
journal, December 2001

Expressed protein ligation: A general method for protein engineering
journal, June 1998

Site-directed conjugation of nonpeptide groups to peptides and proteins via periodate oxidation of a 2-amino alcohol. Application to modification at N-terminal serine
journal, March 1992

Addition of p-Azido-L-phenylalanine to the Genetic Code of Escherichia coli
journal, July 2002

An Expanded Eukaryotic Genetic Code
journal, August 2003

A New Strategy for the Site-Specific Modification of Proteins in Vivo
journal, June 2003

Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
journal, August 2002

A general approach for the generation of orthogonal tRNAs
journal, September 2001

Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli
journal, March 2001

An efficient system for the evolution of aminoacyl-tRNA synthetase specificity
journal, September 2002

Synthesis of Glycoproteins
journal, February 2002

A New Functional Suppressor tRNA/Aminoacyl−tRNA Synthetase Pair for the in Vivo Incorporation of Unnatural Amino Acids into Proteins
journal, May 2000

N-Linked Glycosylation in Campylobacter jejuni and Its Functional Transfer into E. coli
journal, November 2002

Recent Progress in the Solid-phase Synthesis of Glycopeptide
journal, July 2000

Adding l-3-(2-Naphthyl)alanine to the Genetic Code of E. coli
journal, March 2002

Biological roles of oligosaccharides: all of the theories are correct
journal, April 1993

Intein-Mediated Synthesis of Proteins Containing Carbohydrates and Other Molecular Probes
journal, June 2000

Purification and properties of β-mannosyltransferase that synthesizes Man-β-GlcNAc-GlcNAc-pyrophosphoryl-dolichol
journal, October 1986

Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002

Aminooxy-, Hydrazide-, and Thiosemicarbazide-Functionalized Saccharides: Versatile Reagents for Glycoconjugate Synthesis
journal, October 1998

Unnatural amino acids as probes of protein structure and function
journal, December 2000

Expanding the Genetic Code of Escherichia coli
journal, April 2001