Quantitative Proteomic Approaches for Studying Phosphotyrosine Signaling
Protein tyrosine phosphorylation is a fundamental mechanism for controlling many aspects of cellular processes, as well as aspects of human health and diseases. Compared to phosphoserine (pSer) and phosphothreonine (pThr), phosphotyrosine (pTyr) signaling is more tightly regulated, but often more challenging to characterize due to significantly lower level of tyrosine phosphorylation (a relative abundance of 1800:200:1 was estimated for pSer/pThr/pTyr in vertebrate cells[1]). In this review, we outline the recent advances in analytical methodologies for enrichment, identification, and accurate quantitation of tyrosine phosphorylated proteins and peptides using antibody-based technologies, capillary liquid chromatography (LC) coupled with mass spectrometry (MS), and various stable isotope labeling strategies, as well as non-MS-based methods such as protein or peptide array methods. These proteomic technological advances provide powerful tools for potentially understanding signal transduction at the system level and provide a basis for discovering novel drug targets for human diseases. [1] Hunter, T. (1998) The Croonian Lecture 1997. The phosphorylation of proteins on tyrosine: its role in cell growth and disease. Philos. Trans. R. Soc. Lond. B Biol. Sci. 353, 583–605
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 903453
- Report Number(s):
- PNWD-SA-7569; 20496; TRN: US200720%%367
- Journal Information:
- Expert Review of Proteomics, 4(1):13-23, Vol. 4, Issue 1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ABUNDANCE
CHROMATOGRAPHY
DISEASES
MASS SPECTROSCOPY
PEPTIDES
PHOSPHORYLATION
PROTEINS
STABLE ISOTOPES
TARGETS
TYROSINE
VERTEBRATES
tyrosine phosphorylation
cell signaling
immunoprecipitation
mass spectrometry
proteomics
stable isotope labeling
Environmental Molecular Sciences Laboratory