The effects of male age on sperm DNA damage in healthy non-smokers

Schmid, T; Eskenazi, B; Baumgartner, A; Marchetti, F; Young, S; Weldon, R; Anderson, D; Wyrobek, A

doi:10.1093/humrep/del338

The effects of male age on sperm DNA damage in healthy non-smokers

Journal Article · Wed Mar 08 04:00:00 EST 2006 · Human Reproduction

DOI:https://doi.org/10.1093/humrep/del338· OSTI ID:902230

Schmid, T; Eskenazi, B; Baumgartner, A; Marchetti, F; Young, S; Weldon, R; Anderson, D; Wyrobek, A

The trend for men to have children at older ages raises concerns that advancing age may increase the production of genetically defective sperm, increasing the risks of transmitting germ-line mutations. We investigated the associations between male age and sperm DNA damage and the influence of several lifestyle factors in a healthy non-clinical group of 80 non-smokers (age: 22-80) with no known fertility problems using the sperm Comet analyses. The average percent of DNA that migrated out of the sperm nucleus under alkaline electrophoresis increased with age (0.18% per year, p=0.006); but there was no age association for damage measured under neutral conditions (p=0.7). Men who consumed >3 cups coffee per day had {approx}20% higher % tail DNA under neutral but not alkaline conditions compared to men who consumed no caffeine (p=0.005). Our findings indicate that (a) older men have increased sperm DNA damage associated with alkali-labile sites or single-strand DNA breaks, and (b) independent of age, men with substantial daily caffeine consumption have increased sperm DNA damage associated with double-strand DNA breaks. DNA damage in sperm can be converted to chromosomal aberrations and gene mutations after fertilization increasing the risks for developmental defects and genetic diseases among offspring.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA

Sponsoring Organization:: USDOE

DOE Contract Number:: W-7405-ENG-48

OSTI ID:: 902230

Report Number(s):: UCRL-JRNL-219655

Journal Information:: Human Reproduction, Journal Name: Human Reproduction Journal Issue: 1 Vol. 22; ISSN 0268-1161

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
BEVERAGES
CAFFEINE
CHILDREN
CHROMOSOMAL ABERRATIONS
DEFECTS
DISEASES
DNA
DNA DAMAGES
ELECTROPHORESIS
FERTILITY
FERTILIZATION
GENE MUTATIONS
GENETICS
MALES
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SPERMATOZOA

The effects of male age on sperm DNA damage in healthy non-smokers

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