Protein Composition of the Vaccinia Virus Mature Virion

Journal Article · Mon Feb 05 04:00:00 EST 2007 · Virology, 358(1):233-247

DOI:https://doi.org/10.1016/j.virol.2006.08.025· OSTI ID:900917

Resch, Wolfgang; Hixson, Kim K.; Moore, Ronald J.; Lipton, Mary S.; Moss, Bernard

The protein content of vaccinia virus mature virions, purified by rate zonal and isopycnic centrifugation and solubilized by SDS or a solution of urea and thiourea, was determined by the accurate mass and time tag technology which uses both tandem mass spectrometry and Fourier transform-ion cyclotron resonance mass spectrometry to detect tryptic peptides separated by high-resolution liquid chromatography. Eighty vaccinia virus-encoded proteins representing 37% of the 218 genes annotated in the complete genome sequence were detected in at least three analyses. Ten proteins accounted for approximately 80% of the mass, while the least abundant proteins made up 1% or less of the mass. Thirteen identified proteins were not previously reported as components of virions. On the other hand, 8 previously described virion proteins were not detected here, presumably due to technical reasons including small size and hydrophobicity. In addition to vaccinia virus-encoded proteins, 24 host proteins omitting isoforms were detected. The most abundant of these were cytoskeletal proteins, heat shock proteins, and proteins involved in translation.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 900917
• Report Number(s): PNNL-SA-51306; 10790; KP1704020
• Journal Information: Virology, 358(1):233-247, Journal Name: Virology, 358(1):233-247 Journal Issue: 1 Vol. 358; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

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Environmental Molecular Sciences Laboratory