Temporal Changes in Gene Expression in Rainbow Trout Exposed to Ethynyl Estradiol

Journal Article · Sat Nov 25 04:00:00 EST 2006 · Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 145(1):73-85

DOI:https://doi.org/10.1016/j.cbpc.2006.10.011· OSTI ID:899811

Hook, Sharon E; Skillman, Ann D; Small, Jack A; Schultz, Irv R

We examined changes in the genomic response during continuous exposure to the xenoestrogen ethynylestradiol. Isogenic rainbow trout Onorhyncus mykiss were exposed to nominal concentrations of 100 ng/L ethynyl estradiol (EE2) for a period 3 weeks. At fixed time points within the exposure fish were euthanized, livers harvested and RNA extracted. Fluorescently labeled cDNA were generated and hybridized against a commercially available Salmonid array (GRASP project, University of Victoria) spotted with 16,000 cDNA's. The slides were scanned to measure abundance of a given transcript in each sample relative to controls. Data were analyzed via Genespring (Silicon Genetics) to identify a list of up and down regulated genes, and to determine gene clustering patterns that can be used as ''expression signatures''. Gene ontology was determined using the annotation available from the GRASP website. Our analysis indicates each exposure time period generated specific gene expression profiles. Changes in gene expression were best understood by grouping genes by their gene expression profiles rather than examining fold change at a particular time point. Many of the genes commonly used as biomarkers of exposure to xenoestrogens were not induced initially and did not have gene expression profiles typical of the majority of genes with altered expression.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 899811
• Report Number(s): PNWD-SA-7278
• Journal Information: Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 145(1):73-85, Journal Name: Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 145(1):73-85
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ABUNDANCE
ESTRADIOL
Ethynyl estradiol
GENES
GENETICS
RNA
SILICON
TROUT