Biosensing Paraoxon in Simulated Environmental Samples by Immobilized Organophosphorus Hydrolase in Functionalized Mesoporous Silica

Journal Article · Sun Dec 31 23:00:00 EST 2006 · Journal of Environmental Quality, 36(1):233-238

DOI:https://doi.org/10.2134/jeq2006.0216· OSTI ID:897672

Lei, Chenghong; Valenta, Michelle M; Saripalli, Prasad; Ackerman, Eric J

There is a critical need for highly sensitive, cost-effective sensors to conduct ecological analyses for environmental and homeland security related applications. We report here on a method which significantly overcomes this difficulty, and demonstrate its application in a biosensor for aquatic environmental applications. A fast-responding and stable biosensor was developed via immobilization of organophosphorus hydrolase (OPH) in functionalized mesoporous silica (FMS) with pore sizes in tens of nanometers. The sensor was tested for detection of paraoxon in simulated environmental samples, under wide ranging physico-chemical conditions.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 897672
• Report Number(s): PNNL-SA-51296; KP1102010
• Journal Information: Journal of Environmental Quality, 36(1):233-238, Journal Name: Journal of Environmental Quality, 36(1):233-238
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DETECTION
HYDROLASES
SECURITY
SILICA