Studies of multi-heme cytochromes from Geobacter sulfurreducens
The Geobacteraceae family predominates in the reduction of uranium in subsurface environments. We are focusing on the model organism, Geobacter sulfurreducens; its genome contains a large number (>100) of cytochromes c that function in metal reduction pathways. Intensive functional genomics and physiological studies are in progress in Prof. Derek Lovley's laboratory, and the complete genome sequence of this organism has been determined by Methe et al. 2003. We are studying cytochromes from the c{sub 7} family that are required for the reduction of Fe(III). Previously, we expressed in E. coli (Londer et al., 2002) and determined the three-dimensional structure at 1.45 {angstrom} resolution (Pokkuluri et al., 2004a) of the three-heme cytochrome c{sub 7} (PpcA, coded by ORF01023) characterized by Lloyd et al., 2003. Further we identified in the G. sulfurreducens genome ORFs for several of its homologs (Pokkuluri et al., 2004a). Four of the ORFs are the same size as PpcA; three other ORFs are polymers of c{sub 7}-type domains, two of which consist of four domains and one of nine domains, that contain 12 and 27 hemes respectively.
- Research Organization:
- Argonne National Laboratory (ANL), Argonne, IL
- Sponsoring Organization:
- USDOE - Office of Science (SC)
- OSTI ID:
- 895389
- Report Number(s):
- CONF-NABIR2004-36
- Country of Publication:
- United States
- Language:
- English
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Family of cytochrome c{sub 7}-type proteins from Geobacter sulfurreducens : the structure of one cytochrome c{sub 7} at 1.45 {Angstrom} resolution.
Structural characterization of a family of cytochromes c{sub 7} involved in Fe(III) respiration by Geobacter sulfurreducens.