Biostimulation of Iron Reduction and Uranium Immobilization: Microbial and Mineralogical Controls

Conference · Wed Mar 17 04:00:00 EST 2004

OSTI ID:895386

Kostka, Joel E; Petrie, Lainie; North, Nadia; Balkwill, David L; Stucki, Joseph W; Kerkhof, Lee

The overall objective of our project is to understand the microbial and geochemical mechanisms controlling the reduction and immobilization of U(VI) during biostimulation in subsurface sediments of the Field Research Center (FRC) which are cocontaminated with uranium and nitrate. The focus will be on activity of microbial populations (metal- and nitrate-reducing bacteria) and iron minerals which are likely to make strong contributions to the fate of uranium during in situ bioremediation. The project will: (1) quantify the relationships between active members of the microbial communities, iron mineralogy, and nitrogen transformations in the field and in laboratory incubations under a variety of biostimulation conditions, (2) purify and physiologically characterize new model metal-reducing bacteria isolated from moderately acidophilic FRC subsurface sediments, and (3) elucidate the biotic and abiotic mechanisms by which FRC aluminosilicate clay minerals are reduced and dissolved under environmental conditions resembling those during biostimulation. Active microbial communities will be assessed using quantitative molecular techniques along with geochemical measurements to determine the different terminal-electron-accepting pathways. Iron minerals will be characterized using a suite of physical, spectroscopic, and wet chemical methods. Monitoring the activity and composition of the denitrifier community in parallel with denitrification intermediates during nitrate removal will provide a better understanding of the indirect effects of nitrate reduction on uranium speciation. Through quantification of the activity of specific microbial populations and an in-depth characterization of Fe minerals likely to catalyze U sorption/precipitation, we will provide important inputs for reaction-based biogeochemical models which will provide the basis for development of in situ U bioremediation strategies. In collaboration with Jack Istok and Lee Krumholz, we have begun to study the change in microbial community composition of FRC sediments during in situ biostimulation in single well push-pull tests. Microbial communities were stimulated in the acidic subsurface via pH neutralization and addition of electron donor to wells. Examination of sediment chemistry in cores sampled immediately adjacent to treated wells revealed that sediment pH increased substantially (by 1-2 pH units), while nitrate was largely depleted. Following the in situ biostimulation, previously cultured metal-reducing {delta}-Proteobacteria 16S rRNA gene sequences substantially increased from 5% to nearly 40% of clone libraries. Quantitative PCR revealed that Geobacter-type 16S rRNA gene sequences increased in biostimulated sediments by one to two orders of magnitude at two of the four sites tested, thereby corroborating information obtained from clone libraries, and indicating that members of the {delta}-Proteobacteria (including Anaeromyxobacter dehalogenans-related and Geobacter-related organisms) are important metal-reducing bacteria in FRC.

• Research Organization: Florida State University, Tallahassee, FL; University of Illinois, Urbana, IL; Rutgers University, New Brunswick, NJ
• Sponsoring Organization: USDOE - Office of Science (SC)
• OSTI ID: 895386
• Report Number(s): CONF-NABIR2004-33
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BINDING ENERGY
BIOREMEDIATION
CHEMISTRY
DENITRIFICATION
ELECTRONS
GENES
IRON
MINERALOGY
MONITORING
NITRATES
NITROGEN
REMOVAL
SEDIMENTS
TRANSFORMATIONS
URANIUM
VALENCE