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Coherent Anti-Stokes Raman Scattering Microscopy: A Biological Review

Journal Article · · Cytometry. Part A, 69(8):779-791
Microscopic imaging of cells and tissues are generated by the interaction of light with either the sample itself or contrast agents that label the sample. Most contrast agents, however, alter the cell in order to introduce molecular labels, complicating live cell imaging. The interaction of light from multiple laser sources has given rise to microscopy, based on Raman scattering or vibrational resonance, which demonstrates selectivity to specific chemical bonds while cell imaging unmodified live cells. Here, we discuss the nonlinear optical technique of coherent anti-Stokes Raman scattering (CARS) microscopy, its instrumentation, and its status in live cell imaging.
Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-76RL01830
OSTI ID:
894840
Report Number(s):
PNNL-SA-47618; 6898; KP1704020
Journal Information:
Cytometry. Part A, 69(8):779-791, Journal Name: Cytometry. Part A, 69(8):779-791
Country of Publication:
United States
Language:
English

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