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Kinetics of Microbial Reduction of Solid Phase U(VI)

Journal Article · · Environmental Science and Technology, 40(20):6290-6296
DOI:https://doi.org/10.1021/es0608601· OSTI ID:894068
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Sodium boltwoodite (NaUO2SiO3OH ?1.5H2O) was used to assess the kinetics of microbial reduction of solid phase U(VI) by a dissimilatory metal-reducing bacterium (DMRB), Shewanella oneidensis strain MR-1. The bioreduction kinetics was studied with Na-boltwoodite in suspension or within alginate beads. Concentrations of U(VI)tot and cell number were varied to evaluate the coupling of U(VI) dissolution, diffusion, and microbial activity. Batch experiments were performed in a non-growth medium with lactate as electron donor at pH 6.8 buffered with PIPES. Microscopic and spectroscopic analyses with transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), and laser-induced fluorescence spectroscopy (LIFS) collectively indicated that solid phase U(VI) was first dissolved and diffused out of grain interiors before it was reduced on bacterial surfaces and/or within the periplasm. The kinetics of solid phase U(VI) bioreduction was well described by a coupled model of bicarbonate-promoted dissolution of Na-boltwoodite, intraparticle uranyl diffusion, and Monod type bioreduction kinetics with respect to dissolved U(VI) concentration. The results demonstrated the intimate coupling of biological, chemical, and physical processes in microbial reduction of solid phase U(VI).

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-76RL01830
OSTI ID:
894068
Report Number(s):
PNNL-SA-48993; 4691; KP1302000
Journal Information:
Environmental Science and Technology, 40(20):6290-6296, Journal Name: Environmental Science and Technology, 40(20):6290-6296
Country of Publication:
United States
Language:
English

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Related Subjects

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS
ALGINATES
BINDING ENERGY
DIFFUSION
DISSOLUTION
ELECTRONS
Environmental Molecular Sciences Laboratory
FLUORESCENCE SPECTROSCOPY
KINETICS
LACTATES
Na-boltwoodite
intragrain diffusion
microbial reduction
uranium
SILICATE MINERALS
SODIUM
SPECTROSCOPY
STRAINS
TRANSMISSION ELECTRON MICROSCOPY
URANIUM MINERALS
VALENCE