AMT Tag Approach to Proteomic Characterization of Deinococcus Radiodurans and Shewanella Oneidensis
Book
·
OSTI ID:892900
Biology is transitioning from a largely qualitative, mostly descriptive science to a quantitative and ultimately predictive science. Advances in high throughput DNA sequencing have made increasing numbers of genome sequences available and enabled a “systems” level analysis of complex biological organisms. The ability to quantitatively measure the array of proteins, also termed the proteome, in prokaryotic cells and communities of cells is key to understanding microbial systems. This chapter focuses on the utility of the AMT tag mass spectrometric approach used to characterize the proteomes of two microbes, Deinococcus radiodurans and Shewanella oneidensis MR-1.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 892900
- Report Number(s):
- PNNL-SA-42692; 3204; 3203; KP1501021; TRN: US200623%%619
- Resource Relation:
- Related Information: Microbial Proteomics, Methods of Biochemical Analysis, 49:113-134
- Country of Publication:
- United States
- Language:
- English
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