Oxidative Stress and Heat-Shock Responses in Desulfovibrio vulgaris by Genome-Wide Transcriptomic Analysis

Journal Article · Tue May 30 04:00:00 EDT 2006 · Antonie Van Leeuwenhoek, 90:41-55

DOI:https://doi.org/10.1007/s10482-006-9059-9· OSTI ID:889039

Zhang, Weiwen; Culley, David E; Hogan, Mike; Vitiritti, Luigi; Brockman, Fred J

Abstract Sulfate-reducing bacteria, like Desulfovibrio vulgaris have developed a set of reactions allowing them to survive in environments. To obtain further knowledge of the protecting mechanisms employed in D. vulgaris against the oxidative stress and heat shock, we performed a genome-wide transcriptomic analysis to determine the cellular responses to both stimuli. The results showed that 130 genes were responsive to oxidative stress, while 427 genes responsive to heat-shock, respectively. Functional analyses suggested that the genes regulated were involved in a variety of cellular functions. Metabolic analysis showed that amino acid biosynthetic pathways were induced by both oxidative stress and heat shock treatments, while fatty acid metabolism, purine and cofactor biosynthesis were induced by heat shock only. Rubrerythrin gene (rbR) were upregulated by the oxidative stress, suggesting its important role in the oxidative resistance, whereas the expression of rubredoxin oxidoreductase (rbO), superoxide ismutase (sodB) and catalase (katA) genes were not subjected to regulation by oxidative stress in D. vulgaris. In addition, the results showed that thioredoxin reductase (trxB) was responsive to oxidative stress, suggesting the thiol-specific redox system might be involved in oxidative protection in D. vulgaris. Comparison of cellular responses to oxidative stress and heat-shock allowed the identification of 66 genes that showed a similar drastic response to both environmental stimuli, implying that they might be part of the general stress response (GSR) network in D. vulgaris, which was further supported by the finding of a conserved motif upstream these common-responsive genes.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 889039
• Report Number(s): PNNL-SA-45443
• Journal Information: Antonie Van Leeuwenhoek, 90:41-55, Journal Name: Antonie Van Leeuwenhoek, 90:41-55 Vol. 90; ISSN 0003-6072; ISSN ALJMAO
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CATALASE
DESULFOVIBRIO
FUNCTIONALS
GENES
METABOLISM
OXIDOREDUCTASES
PURINES
REGULATIONS
RUBREDOXIN
STIMULI
SULFATE-REDUCING BACTERIA