Use of multi-color fluorescence in situ hybridization to detect sperm aneuploidy and spermatid micronuclei in mice treated with chloral hydrate
- San Jose State Univ., San Jose, CA (United States)
- Lawrence Livermore National Laboratory, CA (United States)
- National Institute of Environmental Health Sciences, Research Triangle Park, NC (United States)
Multi-color fluorescence in situ hybridization (FISH) with multiple DNA probes was applied to detect aneuploidy and micronuclei in the germ cells of 10 week-old B63F{sub 1} male mice. Aneuploidy was evaluated in late-step spermatids using DNA probes specific for chromosomes X, Y and 8. Baseline frequencies (per 10,000 cells) of the hyperhaploidy phenotypes XX8, YY8, XY8, X88, and Y88 were 3.2, 0.0, 0.5, 2.5, and 1.5, respectively, among 4 B6C3F{sub 1} mice. The frequencies of XX8, X88, and -88 were not different from those previously reported with a two-chromosome FISH procedure. FISH analysis in early-step spermatids with pan-centromeric and chromosome-X probes reliably labeled nuclei at rates of {approximately}100% and {approximately}50%, respectively. The baseline frequency of spermatids carrying micronuclei was 1.2 per 1000 cells; few micronuclei contained the centromeric label. These new molecular assays are being applied to investigations of the effects of chloral hydrate at doses of 0, 82.7, 165.4, and 413.5 mg/kg upon male germ cells sampling treated in diakinesis, preleptotene, and as stem cells.
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 88903
- Report Number(s):
- CONF-9405324--
- Journal Information:
- Environmental and Molecular Mutagenesis, Journal Name: Environmental and Molecular Mutagenesis Journal Issue: Suppl.23 Vol. 23; ISSN 0893-6692; ISSN EMMUEG
- Country of Publication:
- United States
- Language:
- English
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