New High Performance Magnet Structures for Bead Based MolecularSeparation
New High Performance Magnet Structures for Bead Based Molecular Separation David Humphries Lawrence Berkeley National Laboratory, D.O.E. Joint Genome Institute Abstract High performance Hybrid magnetic separation technology is under continuing development at the D.O.E. Joint Genome Institute and Lawrence Berkeley National Laboratory for general laboratory and high throughput automated applications. This technology has broad applicability for molecular separation in genomics, proteomics and other areas. It s applicability ranges from large and small scale microtiter plate and flow separation processes to single molecule DNA manipulation. It is currently an enabling purification technology for very high throughput production sequencing at the D.O.E. Joint Genome Institute. This technology incorporates hybrid magnetic structures that combine linear permanent magnet material and ferromagnetic material to produce significantly higher fields and gradients than those of currently available commercial devices. These structures incorporate ferromagnetic poles that can be easily shaped to produce complex field distributions for specialized applications. The higher maximum fields and strong gradients of the hybrid structures result in greater holding forces on magnetized targets that are being processed as well as faster extraction. Current development versions of these magnet plates have exhibited fields in excess of 1.0 tesla and gradients approaching 1000.0 tesla/meter. Second generation Hybrid magnet plates have now been developed for both 384 and 96-well applications. This technology is currently being made available to industry through the Tech Transfer Department at Lawrence Berkeley National Laboratory. This work was performed under the auspices of the US Department of Energy's Office of Science, Biological and Environmental Research Program and the by the University of California, Lawrence Livermore National Laboratory under Contract No. W-7405-Eng-48, Lawrence Berkeley National Laboratory under contract No. DE-AC03-6SF00098 and Los Alamos National Laboratory under contract No. W-7405-ENG-36.
- Research Organization:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE Director. Office of Science. Office of Biological andEnvironmental Research, Lawrence Livermore National Laboratory, LosAlamos National Laboratory
- DOE Contract Number:
- DE-AC02-05CH11231
- OSTI ID:
- 883775
- Report Number(s):
- LBNL-58894; R&D Project: 625989; BnR: KP1103010; TRN: US200615%%233
- Journal Information:
- Bioscienceworld -- Laboratory Focus, Vol. none, Issue none; Related Information: Journal Publication Date: 2006
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
FERROMAGNETIC MATERIALS
MAGNETS
PERFORMANCE
PERMANENT MAGNETS
PLATES
PRODUCTION
PURIFICATION
RESEARCH PROGRAMS
SEPARATION PROCESSES
TARGETS
Magnet plates hybrid magnets molecular extraction magneticseparation magnetic purification bead based magnetic separation magneticbeads molecular separation