Systems For Column-Based Separations, Methods Of Forming Packed Columns, And Methods Of Purifying Sample Components

Patent · Tue Feb 21 00:00:00 EST 2006

OSTI ID:880479

Egorov, Oleg B ^[1]; O'Hara, Matthew J ^[1]; Grate, Jay W ^[2]; Chandler, Darrell P ^[1]; Brockman, Fred J ^[3]; Bruckner-Lea, Cynthia J ^[1]

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1. Richland, WA
2. West Richland, WA
3. Kennewick, WA

The invention encompasses systems for column-based separations, methods of packing and unpacking columns and methods of separating components of samples. In one aspect, the invention includes a method of packing and unpacking a column chamber, comprising: a) packing a matrix material within a column chamber to form a packed column; and b) after the packing, unpacking the matrix material from the column chamber without moving the column chamber. In another aspect, the invention includes a system for column-based separations, comprising: a) a fluid passageway, the fluid passageway comprising a column chamber and a flow path in fluid communication with the column chamber, the flow path being obstructed by a retaining material permeable to a carrier fluid and impermeable to a column matrix material suspended in the carrier fluid, the flow path extending through the column chamber and through the retaining material, the flow path being configured to form a packed column within the column chamber when a suspension of the fluid and the column matrix material is flowed along the flow path; and b) the fluid passageway extending through a valve intermediate the column chamber and the retaining material.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
• DOE Contract Number: AC06-76RL01830
• Assignee: Battelle Memorial Institute (Richland, WA)
• Patent Number(s): US 7,001,522
• Application Number: 10/857388
• OSTI ID: 880479
• Country of Publication: United States
• Language: English