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Title: Selective Destruction Of Cells Infected With The Human Immunodeficiency Virus

Abstract

Compositions and methods for selectively killing a cell containing a viral protease are disclosed. The composition is a varient of a protein synthesis inactivating toxin wherein a viral protease cleavage site is interposed between the A and B chains. The variant of the type II ribosome-inactivating protein is activated by digestion of the viral protease cleavage site by the specific viral protease. The activated ribosome-inactivating protein then kills the cell by inactivating cellular ribosomes. A preferred embodiment of the invention is specific for human immunodeficiency virus (HIV) and uses ricin as the ribosome-inactivating protein. In another preferred embodiment of the invention, the variant of the ribosome-inactivating protein is modified by attachment of one or more hydrophobic agents. The hydrophobic agent facilitates entry of the variant of the ribosome-inactivating protein into cells and can lead to incorporation of the ribosome-inactivating protein into viral particles. Still another preferred embodiment of the invention includes a targeting moiety attached to the variants of the ribosome-inactivating protein to target the agent to HIV infectable cells.

Inventors:
 [1];  [1]
  1. (Idaho Falls, ID)
Publication Date:
Research Org.:
LOCKHEED MARTIN IDAHO TECH CO; BECHTEL BWXT IDAHO LLC; Idaho National Lab. (INL), Idaho Falls, ID (United States)
OSTI Identifier:
880117
Patent Number(s):
US 7,018,633
Application Number:
10/618560
Assignee:
Battelle Energy Alliance, LLC (Idaho Falls, ID) IDO
DOE Contract Number:
AC07-94ID13223; AC07-99ID13727; AC07-05ID14517
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Keener, William K., and Ward, Thomas E.. Selective Destruction Of Cells Infected With The Human Immunodeficiency Virus. United States: N. p., 2006. Web.
Keener, William K., & Ward, Thomas E.. Selective Destruction Of Cells Infected With The Human Immunodeficiency Virus. United States.
Keener, William K., and Ward, Thomas E.. Tue . "Selective Destruction Of Cells Infected With The Human Immunodeficiency Virus". United States. doi:. https://www.osti.gov/servlets/purl/880117.
@article{osti_880117,
title = {Selective Destruction Of Cells Infected With The Human Immunodeficiency Virus},
author = {Keener, William K. and Ward, Thomas E.},
abstractNote = {Compositions and methods for selectively killing a cell containing a viral protease are disclosed. The composition is a varient of a protein synthesis inactivating toxin wherein a viral protease cleavage site is interposed between the A and B chains. The variant of the type II ribosome-inactivating protein is activated by digestion of the viral protease cleavage site by the specific viral protease. The activated ribosome-inactivating protein then kills the cell by inactivating cellular ribosomes. A preferred embodiment of the invention is specific for human immunodeficiency virus (HIV) and uses ricin as the ribosome-inactivating protein. In another preferred embodiment of the invention, the variant of the ribosome-inactivating protein is modified by attachment of one or more hydrophobic agents. The hydrophobic agent facilitates entry of the variant of the ribosome-inactivating protein into cells and can lead to incorporation of the ribosome-inactivating protein into viral particles. Still another preferred embodiment of the invention includes a targeting moiety attached to the variants of the ribosome-inactivating protein to target the agent to HIV infectable cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 28 00:00:00 EST 2006},
month = {Tue Mar 28 00:00:00 EST 2006}
}

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