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Title: Fluid delivery system for capillary electrophoretic applications

Abstract

An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.

Inventors:
; ; ; ; ;
Publication Date:
Research Org.:
Spectrumedix Corporation, State College, PA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
879604
Patent Number(s):
6,375,819
Application Number:
09/373,909
Assignee:
Spectrumedix Corporation (State College, PA)
DOE Contract Number:  
FG02-97ER82461
Resource Type:
Patent
Resource Relation:
Patent File Date: 1999 Aug 13
Country of Publication:
United States
Language:
English

Citation Formats

Li, Qingbo, Liu, Changsheng, Kane, Thomas E., Kernan, John R., Sonnenschein, Bernard, and Sharer, Michael V. Fluid delivery system for capillary electrophoretic applications. United States: N. p., 2002. Web.
Li, Qingbo, Liu, Changsheng, Kane, Thomas E., Kernan, John R., Sonnenschein, Bernard, & Sharer, Michael V. Fluid delivery system for capillary electrophoretic applications. United States.
Li, Qingbo, Liu, Changsheng, Kane, Thomas E., Kernan, John R., Sonnenschein, Bernard, and Sharer, Michael V. 2002. "Fluid delivery system for capillary electrophoretic applications". United States. https://www.osti.gov/servlets/purl/879604.
@article{osti_879604,
title = {Fluid delivery system for capillary electrophoretic applications},
author = {Li, Qingbo and Liu, Changsheng and Kane, Thomas E. and Kernan, John R. and Sonnenschein, Bernard and Sharer, Michael V.},
abstractNote = {An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.},
doi = {},
url = {https://www.osti.gov/biblio/879604}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {4}
}