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Title: Fluid Delivery System For Capillary Electrophoretic Applications.

Abstract

An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.

Inventors:
 [1];  [1];  [1];  [2];  [3];  [4]
  1. (State College, PA)
  2. (Loganton, PA)
  3. (Brooklyn, NY)
  4. (Tyrone, PA)
Publication Date:
Research Org.:
Spectrumedix Corporation (State College, PA)
OSTI Identifier:
879604
Patent Number(s):
US 6375819
Application Number:
09/373909
Assignee:
Spectrumedix Corporation (State College, PA) CHO
DOE Contract Number:  
FG02-97ER82461
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Li, Qingbo, Liu, Changsheng, Kane, Thomas E., Kernan, John R., Sonnenschein, Bernard, and Sharer, Michael V. Fluid Delivery System For Capillary Electrophoretic Applications.. United States: N. p., 2002. Web.
Li, Qingbo, Liu, Changsheng, Kane, Thomas E., Kernan, John R., Sonnenschein, Bernard, & Sharer, Michael V. Fluid Delivery System For Capillary Electrophoretic Applications.. United States.
Li, Qingbo, Liu, Changsheng, Kane, Thomas E., Kernan, John R., Sonnenschein, Bernard, and Sharer, Michael V. Tue . "Fluid Delivery System For Capillary Electrophoretic Applications.". United States. https://www.osti.gov/servlets/purl/879604.
@article{osti_879604,
title = {Fluid Delivery System For Capillary Electrophoretic Applications.},
author = {Li, Qingbo and Liu, Changsheng and Kane, Thomas E. and Kernan, John R. and Sonnenschein, Bernard and Sharer, Michael V.},
abstractNote = {An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {4}
}

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