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Title: Multiple tag labeling method for DNA sequencing

Abstract

A DNA sequencing method is described which uses single lane or channel electrophoresis. Sequencing fragments are separated in the lane and detected using a laser-excited, confocal fluorescence scanner. Each set of DNA sequencing fragments is separated in the same lane and then distinguished using a binary coding scheme employing only two different fluorescent labels. Also described is a method of using radioisotope labels. 5 figs.

Inventors:
; ;
Publication Date:
Research Org.:
University of California
OSTI Identifier:
87755
Patent Number(s):
US 5,436,130/A/
Application Number:
PAN: 8-143,377
Assignee:
Univ. of California, Oakland, CA (United States) PTO; SCA: 550200; PA: EDB-95:120062; SN: 95001431579
DOE Contract Number:  
FG03-91ER61125
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 25 Jul 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCERS; DESIGN; DNA SEQUENCING; TRACER TECHNIQUES; RADIOISOTOPES; LABELLING

Citation Formats

Mathies, R.A., Huang, X.C., and Quesada, M.A. Multiple tag labeling method for DNA sequencing. United States: N. p., 1995. Web.
Mathies, R.A., Huang, X.C., & Quesada, M.A. Multiple tag labeling method for DNA sequencing. United States.
Mathies, R.A., Huang, X.C., and Quesada, M.A. Tue . "Multiple tag labeling method for DNA sequencing". United States. doi:.
@article{osti_87755,
title = {Multiple tag labeling method for DNA sequencing},
author = {Mathies, R.A. and Huang, X.C. and Quesada, M.A.},
abstractNote = {A DNA sequencing method is described which uses single lane or channel electrophoresis. Sequencing fragments are separated in the lane and detected using a laser-excited, confocal fluorescence scanner. Each set of DNA sequencing fragments is separated in the same lane and then distinguished using a binary coding scheme employing only two different fluorescent labels. Also described is a method of using radioisotope labels. 5 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 25 00:00:00 EDT 1995},
month = {Tue Jul 25 00:00:00 EDT 1995}
}