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Multiple tag labeling method for DNA sequencing

Patent ·
OSTI ID:87755
A DNA sequencing method is described which uses single lane or channel electrophoresis. Sequencing fragments are separated in the lane and detected using a laser-excited, confocal fluorescence scanner. Each set of DNA sequencing fragments is separated in the same lane and then distinguished using a binary coding scheme employing only two different fluorescent labels. Also described is a method of using radioisotope labels. 5 figs.
Research Organization:
University of California
DOE Contract Number:
FG03-91ER61125
Assignee:
Univ. of California, Oakland, CA (United States)
Patent Number(s):
US 5,436,130/A/
Application Number:
PAN: 8-143,377
OSTI ID:
87755
Country of Publication:
United States
Language:
English

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