Microfluidic devices for the controlled manipulation of small volumes
Patent
·
OSTI ID:875072
- Knoxville, TN
A method for conducting a broad range of biochemical analyses or manipulations on a series of nano- to subnanoliter reaction volumes and an apparatus for carrying out the same are disclosed. The method and apparatus are implemented on a fluidic microchip to provide high serial throughput. The method and device of the invention also lend themselves to multiple parallel analyses and manipulation to provide greater throughput for the generation of biochemical information. In particular, the disclosed device is a microfabricated channel device that can manipulate nanoliter or subnanoliter biochemical reaction volumes in a controlled manner to produce results at rates of 1 to 10 Hz per channel. The individual reaction volumes are manipulated in serial fashion analogous to a digital shift register. The method and apparatus according to this invention have application to such problems as screening molecular or cellular targets using single beads from split-synthesis combinatorial libraries, screening single cells for RNA or protein expression, genetic diagnostic screening at the single cell level, or performing single cell signal transduction studies.
- Research Organization:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-00OR22725
- Assignee:
- UT-Battelle, LLC (Oak Ridge, TN)
- Patent Number(s):
- 6,524,456
- OSTI ID:
- 875072
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
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10
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chemical reaction
combinatorial
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fluidic
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lend
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libraries
manipulate
manipulated
manipulation
manipulations
manner
method
microchip
microfabricated
microfluidic
microfluidic devices
molecular
multiple
nano-
nanoliter
parallel
performing
produce
protein
provide
range
rates
reaction
reaction volume
register
results
rna
screening
serial
series
shift
signal
single
single cell
split-synthesis
studies
subnanoliter
targets
throughput
transduction
volumes
10
analogous
analyses
apparatus
application
beads
biochemical
broad
carrying
cell
cells
cellular
channel
chemical reaction
combinatorial
conducting
controlled
device
devices
diagnostic
digital
disclosed
expression
fashion
fluidic
generation
genetic
hz
implemented
individual
information
lend
level
libraries
manipulate
manipulated
manipulation
manipulations
manner
method
microchip
microfabricated
microfluidic
microfluidic devices
molecular
multiple
nano-
nanoliter
parallel
performing
produce
protein
provide
range
rates
reaction
reaction volume
register
results
rna
screening
serial
series
shift
signal
single
single cell
split-synthesis
studies
subnanoliter
targets
throughput
transduction
volumes