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DNA attachment to support structures

Patent ·
OSTI ID:874582
 [1];  [2]
  1. Livermore, CA
  2. Fresno, CA
+ Show Author Affiliations

Microscopic beads or other structures are attached to nucleic acids (DNA) using a terminal transferase. The transferase adds labeled dideoxy nucleotide bases to the ends of linear strands of DNA. The labels, such as the antigens digoxigenin and biotin, bind to the antibody compounds or other appropriate complementary ligands, which are bound to the microscopic beads or other support structures. The method does not require the synthesis of a synthetic oligonucleotide probe. The method can be used to tag or label DNA even when the DNA has an unknown sequence, has blunt ends, or is a very large fragment (e.g., >500 kilobase pairs).

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA
DOE Contract Number:
W-7405-ENG-48
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
US 6420112
OSTI ID:
874582
Country of Publication:
United States
Language:
English

References (6)

Overstretching B-DNA: The Elastic Response of Individual Double-Stranded and Single-Stranded DNA Molecules journal February 1996
Deoxynucleotide-polymerizing Enzymes of Calf Thymus Gland journal June 1967
The telomere terminal transferase of tetrahymena is a ribonucleoprotein enzyme with two kinds of primer specificity journal December 1987
Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads journal November 1992
Ionic effects on the elasticity of single DNA molecules journal June 1997
Behavior of Supercoiled DNA journal April 1998

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Related Subjects

/435/436/530/999/
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