Geranyl diphosphate synthase large subunit, and methods of use

Patent · Sun Dec 31 23:00:00 EST 2000

OSTI ID:874056

Croteau, Rodney B ^[1]; Burke, Charles C ^[2]; Wildung, Mark R ^[3]

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1. Pullman, WA
2. Moscow, ID
3. Colfax, WA

A cDNA encoding geranyl diphosphate synthase large subunit from peppermint has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Replicable recombinant cloning vehicles are provided which code for geranyl diphosphate synthase large subunit). In another aspect, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranyl diphosphate synthase large subunit. In yet another aspect, the present invention provides isolated, recombinant geranyl diphosphate synthase protein comprising an isolated, recombinant geranyl diphosphate synthase large subunit protein and an isolated, recombinant geranyl diphosphate synthase small subunit protein. Thus, systems and methods are provided for the recombinant expression of geranyl diphosphate synthase.

• Research Organization: Washington State University Research Foundation (Pullma, WA)
• DOE Contract Number: FG03-96ER20212
• Assignee: Washington State University Research Foundation (Pullma, WA)
• Patent Number(s): US 6303330
• Application Number: 09/420211
• OSTI ID: 874056
• Country of Publication: United States
• Language: English

Related Subjects

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acid
acid sequence
amino
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andor
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cdna
cells
cloning
cloning vehicle
code
comprising
corresponding amino
determined
diphosphate
dna
dna encoding
encoding
expression
geranyl
host
infected
injected
isolated
methods
modified
peppermint
protein
provided
provides
recombinant
replicable
sequence
sequenced
subunit
synthase
systems
transfected
transformed
vehicle
vehicles