Geranyl diphosphate synthase large subunit, and methods of use

Patent · Mon Jan 01 00:00:00 EST 2001

OSTI ID:874056

Croteau, Rodney B ^[1]; Burke, Charles C ^[2]; Wildung, Mark R ^[3]

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1. Pullman, WA
2. Moscow, ID
3. Colfax, WA

A cDNA encoding geranyl diphosphate synthase large subunit from peppermint has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Replicable recombinant cloning vehicles are provided which code for geranyl diphosphate synthase large subunit). In another aspect, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranyl diphosphate synthase large subunit. In yet another aspect, the present invention provides isolated, recombinant geranyl diphosphate synthase protein comprising an isolated, recombinant geranyl diphosphate synthase large subunit protein and an isolated, recombinant geranyl diphosphate synthase small subunit protein. Thus, systems and methods are provided for the recombinant expression of geranyl diphosphate synthase.

• Research Organization: Washington State University Research Foundation (Pullma, WA)
• DOE Contract Number: FG03-96ER20212
• Assignee: Washington State University Research Foundation (Pullma, WA)
• Patent Number(s): US 6303330
• Application Number: 09/420211
• OSTI ID: 874056
• Country of Publication: United States
• Language: English

Related Subjects

geranyl
diphosphate
synthase
subunit
methods
cdna
encoding
peppermint
isolated
sequenced
amino
acid
sequence
determined
replicable
recombinant
cloning
vehicles
provided
code
aspect
modified
host
cells
transformed
transfected
infected
andor
injected
vehicle
dna
provides
protein
comprising
systems
expression
amino acid
acid sequence
cloning vehicle
dna encoding
corresponding amino
/435/999/