Waveguide detection of right-angle-scattered light in flow cytometry

Patent · Fri Dec 31 23:00:00 EST 1999

OSTI ID:873418

A transparent flow cell is used as an index-guided optical waveguide. A detector for the flow cell but not the liquid stream detects the Right-Angle-Scattered (RAS) Light exiting from one end of the flow cell. The detector(s) could view the trapped RAS light from the flow cell either directly or through intermediate optical light guides. If the light exits one end of the flow cell, then the other end of the flow cell can be given a high-reflectivity coating to approximately double the amount of light collected. This system is more robust in its alignment than the traditional flow cytometry systems which use imaging optics, such as microscope objectives.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA
• DOE Contract Number: W-7405-ENG-48
• Assignee: Regents of University of California (Oakland, CA)
• Patent Number(s): US 6154276
• OSTI ID: 873418
• Country of Publication: United States
• Language: English

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alignment
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approximately
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cytometry
detection
detector
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directly
double
exiting
exits
flow
flow cell
flow cytometry
guides
high-reflectivity
imaging
imaging optics
index-guided
intermediate
light
light collected
light guide
light guides
liquid
liquid stream
microscope
microscope objective
objectives
optical
optical light
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optical waveguide
optics
ras
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right-angle-scattered light
robust
scattered light
stream
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transparent
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waveguide